研究のタイプ: 医学／生物学の研究 (experimental study)

[2種類のヒト初代内皮細胞における携帯電話放射へのプロテオーム反応の分析] med./bio.

Analysis of proteome response to the mobile phone radiation in two types of human primary endothelial cells

著者: Nylund R, Kuster N, Leszczynski D
掲載誌: Proteome Sci 2010; 8 (1): 52
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この研究は、ヒトの初代内皮細胞プロテオームに対する1800MHz GSM携帯電話放射へのばく露の影響を調べた。用いた細胞は、ヒト臍静脈の初代内皮細胞とヒトの脳の初代微小血管内皮細胞であり、ばく露は平均SAR 2.0W/kgでばく露時間は1時間とした。ばく露終了後、ばく露群と擬似ばく露群の細胞の蛋白質発現パターンを二次元ゲル電気泳動法に基づくプロテオミクス（2DE-DIGE法）で分析した。その結果、擬似ばく露群において2つの初代内皮細胞のプロテオームに多数の違いが認められた；これらの違いは異なる血管床の内皮細胞の生理学的差異を表すと考えてほぼ間違いない； 2つの初代内皮細胞とも、ばく露による蛋白質発現の有意な変化を示さなかった、と報告している。

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研究目的（著者による）

To study whether exposure to 1800 MHz GSM mobile phone irradiation can affect cell proteome of two types of human primary endothelial cells (primary cell cultures).

詳細情報

Primary human umbilical vein endothelial cells (pooled from different donors) and primary human brain microvascular endothelial cells (from a single donor) were used and examined immediately after exposure. 13 independent sham exposed and exposed samples were generated from human umbilical vein endothelial cells and 11 independent sham exposed and exposed samples were generated from human brain microvascular endothelial cells.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: pulsed
ばく露時間: continuous for 1 h

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 h
Modulation
Modulation type pulsed
Additional information

modulation frequencies: 2, 8, 217, 1733 Hz mean duration of 10.8 seconds for non-DTX ("talking") and 5.6 seconds for DTX ("listening")
ばく露装置
ばく露の発生源／構造
  • simulated GSM Signal
ばく露装置の詳細 The system consisted of two identical exposure chambers mounted inside the same cell culture incubator. One of the chambers acted as a sham exposure and the other as an experimental chamber.
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg - 測定値 - -

Reference articles

  • Schuderer J et al. (2004): [1800 MHzのインビトロ実験に用いる強力なばく露および環境制御機能を備えた高尖頭SARばく露装置]
  • Schönborn F et al. (2001): [移動通信の健康ハザード評価にためのIn vitroばく露装置の最適化]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

The data showed numerous differences (368 protein spots) between the proteomes of sham exposed human umbilical vein endothelial cells and human brain microvascular endothelial cells. These differences are most likely representing physiological differences between endothelia in different vascular beds. However, the exposure of both types of primary endothelial cells to mobile phone irradiation did not cause any statistically significant changes in protein expression.
In conclusions, exposure of primary human endothelial cells to the mobile phone irradiation at 1800 MHz GSM signal for 1 hour (SAR of 2 W/kg) did not affect protein expression, when the proteomes were examined immediately after the end of the exposure and when the false discovery rate correction was applied to analysis. This observation agrees with earlier studies of the authors showing that the 1800 MHz GSM exposure had only very limited effect on the proteome of human endothelial cell line EA.hy926 (Nylund et al. 2009), as compared with the effect of 900 MHz GSM exposure (Nylund et al. 2006).

研究の種別:

研究助成

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