この研究は、ヒトの初代内皮細胞プロテオームに対する1800MHz GSM携帯電話放射へのばく露の影響を調べた。用いた細胞は、ヒト臍静脈の初代内皮細胞とヒトの脳の初代微小血管内皮細胞であり、ばく露は平均SAR 2.0W/kgでばく露時間は1時間とした。ばく露終了後、ばく露群と擬似ばく露群の細胞の蛋白質発現パターンを二次元ゲル電気泳動法に基づくプロテオミクス（2DE-DIGE法）で分析した。その結果、擬似ばく露群において2つの初代内皮細胞のプロテオームに多数の違いが認められた；これらの違いは異なる血管床の内皮細胞の生理学的差異を表すと考えてほぼ間違いない； 2つの初代内皮細胞とも、ばく露による蛋白質発現の有意な変化を示さなかった、と報告している。
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Primary human umbilical vein endothelial cells (pooled from different donors) and primary human brain microvascular endothelial cells (from a single donor) were used and examined immediately after exposure. 13 independent sham exposed and exposed samples were generated from human umbilical vein endothelial cells and 11 independent sham exposed and exposed samples were generated from human brain microvascular endothelial cells.
Modulation type: pulsed
ばく露時間: continuous for 1 h
|ばく露時間||continuous for 1 h|
modulation frequencies: 2, 8, 217, 1733 Hz mean duration of 10.8 seconds for non-DTX ("talking") and 5.6 seconds for DTX ("listening")
|ばく露装置の詳細||The system consisted of two identical exposure chambers mounted inside the same cell culture incubator. One of the chambers acted as a sham exposure and the other as an experimental chamber.|
|Sham exposure||A sham exposure was conducted.|
The data showed numerous differences (368 protein spots) between the proteomes of sham exposed human umbilical vein endothelial cells and human brain microvascular endothelial cells. These differences are most likely representing physiological differences between endothelia in different vascular beds. However, the exposure of both types of primary endothelial cells to mobile phone irradiation did not cause any statistically significant changes in protein expression.
In conclusions, exposure of primary human endothelial cells to the mobile phone irradiation at 1800 MHz GSM signal for 1 hour (SAR of 2 W/kg) did not affect protein expression, when the proteomes were examined immediately after the end of the exposure and when the false discovery rate correction was applied to analysis. This observation agrees with earlier studies of the authors showing that the 1800 MHz GSM exposure had only very limited effect on the proteome of human endothelial cell line EA.hy926 (Nylund et al. 2009), as compared with the effect of 900 MHz GSM exposure (Nylund et al. 2006).