研究のタイプ: 医学/生物学の研究 (experimental study)

[Balb/c 3T3細胞の細胞挙動に対する長期の50Hz電力周波電磁界の影響] med./bio.

Effects of long-term 50 Hz power-line frequency electromagnetic field on cell behavior in Balb/c 3T3 cells.

掲載誌: PLoS One 2015; 10 (2): e0117672

この研究は、指数関数的増殖期のBalb/c 3T3細胞に、50Hz電力周波磁界(2.3mT)を1日2時間、週5日、11週間ばく露(または擬似ばく露)して、ばく露終了後のそれらの細胞の挙動を調べた。その結果、細胞の形態、アポトーシス細胞遊走能、細胞形質転換には、ばく露による変化は見られなかった;その一方、擬似ばく露群に比べ、ばく露群の細胞生存率は大きく低下し、細胞分裂周期分布も変化した;また、増殖細胞核抗原(PCNA)およびサイクリンD1のタンパク質の濃度は有意に低下した、と報告している。

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研究目的(著者による)

To investigate the effects of long-term 50 Hz magnetic field exposure on cell behavior (cell proliferation, cell viability, cell cycle, cell transformation, cell morphology) of mouse fibroblasts.

詳細情報

Mouse fibroblasts were exposed or sham exposed during the exponential growth phase. Rat glioma cells (C6 cells) were used as a positive control in the plate assay and human esophageal squamous cell carcinoma cells (KYSE150 cells) were used as a positive control in the soft agar assay.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: 2 h/day, 5 days/week for 11 weeks

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 2 h/day, 5 days/week for 11 weeks
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 setup consisted of 2 four-coil systems each placed inside a mu-metal box, respectively; both systems were inside a commercial incubator; temperature was 37°C; atmosphere consisted of 95% air, 5% CO2 and 100% relative humidity; temperature was monitored at the location of the flasks during exposure with Pt100 probes; two fans per box were mounted to guarantee enough atmospheric exchange of the exposure chambers
Sham exposure A sham exposure was conducted.
Additional information current in the bifilar coils could be switched parallel for field exposure or non-parallel for sham exposure
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2.3 mT effective value 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

No significant changes were observed between exposed and sham exposed mouse fibroblasts regarding cell morphology, apoptosis, cell migration ability (scratch test) and cell transformation. However, the cell viability and the protein expression of PCNA and CyclinD1 were significantly decreased in the exposed group compared to the sham exposure. Additionally, the cell cycle distribution was significantly changed: After eleven weeks of exposure, the percentage of cells in the G1 phase and in the G2 phase was increased compared to the sham exposed cells while the percentage of cells in the S phase was decreased.
The authors conclude that long-term 50 Hz magnetic field exposure could affect cell viability and cell cycle by down-regulation of the proteins PCNA and CyclinD1.

研究の種別:

研究助成

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