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To investigate the effects of long-term 50 Hz magnetic field exposure on cell behavior (cell proliferation, cell viability, cell cycle, cell transformation, cell morphology) of mouse fibroblasts.
Mouse fibroblasts were exposed or sham exposed during the exponential growth phase. Rat glioma cells (C6 cells) were used as a positive control in the plate assay and human esophageal squamous cell carcinoma cells (KYSE150 cells) were used as a positive control in the soft agar assay.
ばく露時間: 2 h/day, 5 days/week for 11 weeks
|ばく露時間||2 h/day, 5 days/week for 11 weeks|
|ばく露装置の詳細||setup consisted of 2 four-coil systems each placed inside a mu-metal box, respectively; both systems were inside a commercial incubator; temperature was 37°C; atmosphere consisted of 95% air, 5% CO2 and 100% relative humidity; temperature was monitored at the location of the flasks during exposure with Pt100 probes; two fans per box were mounted to guarantee enough atmospheric exchange of the exposure chambers|
|Sham exposure||A sham exposure was conducted.|
|Additional information||current in the bifilar coils could be switched parallel for field exposure or non-parallel for sham exposure|
|磁束密度||2.3 mT||effective value||測定値||-||-|
No significant changes were observed between exposed and sham exposed mouse fibroblasts regarding cell morphology, apoptosis, cell migration ability (scratch test) and cell transformation. However, the cell viability and the protein expression of PCNA and CyclinD1 were significantly decreased in the exposed group compared to the sham exposure. Additionally, the cell cycle distribution was significantly changed: After eleven weeks of exposure, the percentage of cells in the G1 phase and in the G2 phase was increased compared to the sham exposed cells while the percentage of cells in the S phase was decreased.
The authors conclude that long-term 50 Hz magnetic field exposure could affect cell viability and cell cycle by down-regulation of the proteins PCNA and CyclinD1.