研究のタイプ: 医学／生物学の研究 (experimental study)

[900 MHzの電磁界のT-リンパ芽球性白血病CCRF-CEM細胞へのばく露は、プロアポトーシスと生存促進シグナル間のアンバランスを誘発する] med./bio.

Exposure to 900 MHz electromagnetic field induces an unbalance between pro-apoptotic and pro-survival signals in T-lymphoblastoid leukemia CCRF-CEM cells

著者: Marinelli F, La Sala D, Cicciotti G, Cattini L, Trimarchi C, Putti S, Zamparelli A, Giuliani L, Tomassetti G, Cinti C
掲載誌: J Cell Physiol 2004; 198 (2): 324-332
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Tリンパ芽白血病CCRF‐CEM細胞への900MHz電磁場暴露は,プロアポトーシスとプロ生存シグナル間の不均衡を誘導する

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研究目的（著者による）

To inverstigate the effect of unmodulated high-frequency electromagnetic field at various exposure times on in vitro survival of cultured acute human T-lymphoblastoid leukemia cells.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: CW
ばく露時間: continuous for 2, 4, 12, 24, and 48 h

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 2, 4, 12, 24, and 48 h
Additional information Stuchly MA, Stuchly SS, 1996. Experimental radio and microwave dosimetry. In: Polk C, Postow E, editors. Biological effects of electromagnetic fields. New York: CRC press, Inc. pp 295-336.
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
チャンバの詳細 The TEM cell placed inside an incubator (37°C, 95% humidity and 5% CO2) was a 3 mm copper box of 19.25 x 20 x 50 cm with a tapered horizontal copper septum dividing the inner space into two equal parts. To allow gaseous exchange between the incubator and the cell cultures, one side of TEM wall was made of a 2.5-mm size copper-mesh. The geometry and field propagation have been well described in literature [Stuchly and Stuchly, 1996]. The electric field was perpendicular to the septum plane.
ばく露装置の詳細 Five Petri dishes of 10 cm diameter containing 20 ml of medium were placed in the TEM cell at 1 cm distance above and below the septum.
Sham exposure A sham exposure was conducted.
Additional information Continuous temperature monitoring detected no more than 0.15°C of difference. Sham exposure was performed for 2, 24, and 48 h comparing cells inside and outside the TEM cell.
パラメータ
測定量 値 種別 Method Mass 備考
電界強度 2.96 V/m - 測定値 - -
磁界強度 8 mA/m - 測定値 - -
電力密度 23.68 mW/m² - - - -
SAR 3.5 mW/kg - - - -
Additional parameter details

50 Hz electric and magnetic field did not exceed 0.8 V/m and 0.16 µT, respectively.

測定および計算の詳細

The magnetic and electric field in the TEM cell were measured using a spectrum analyser equipped with a 1-cm magnetic loop or a 3-cm electrospherical probe. Detected values matched the theoretically expected values within the systematic error range. A directional coupler, an RF detector, and a scalar meter with a CRT display were used to optimise and continuously check the input return loss of the TEM cell. Properly adjusted, the system measured a return loss of 27 dB with and without the Petri dishes inside resulting in a field uniformity of better than 2 dB.

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

At short time exposure (2-12 h) early activation of both p53-dependend and independend apoptotic pathways, and an induction of DNA breaks were observed.
In contrast, long time exposure (24-48 h) leads to the activation of pro-survival Ras pathways and an increased DNA synthesis.
These findings suggest that 900 MHz EMF exposure is capable to induce genotoxic damage and changes in gene expression.

研究の種別:

研究助成

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