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To inverstigate the effect of unmodulated high-frequency electromagnetic field at various exposure times on in vitro survival of cultured acute human T-lymphoblastoid leukemia cells.
|ばく露時間||continuous for 2, 4, 12, 24, and 48 h|
|Additional information||Stuchly MA, Stuchly SS, 1996. Experimental radio and microwave dosimetry. In: Polk C, Postow E, editors. Biological effects of electromagnetic fields. New York: CRC press, Inc. pp 295-336.|
|チャンバの詳細||The TEM cell placed inside an incubator (37°C, 95% humidity and 5% CO2) was a 3 mm copper box of 19.25 x 20 x 50 cm with a tapered horizontal copper septum dividing the inner space into two equal parts. To allow gaseous exchange between the incubator and the cell cultures, one side of TEM wall was made of a 2.5-mm size copper-mesh. The geometry and field propagation have been well described in literature [Stuchly and Stuchly, 1996]. The electric field was perpendicular to the septum plane.|
|ばく露装置の詳細||Five Petri dishes of 10 cm diameter containing 20 ml of medium were placed in the TEM cell at 1 cm distance above and below the septum.|
|Sham exposure||A sham exposure was conducted.|
|Additional information||Continuous temperature monitoring detected no more than 0.15°C of difference. Sham exposure was performed for 2, 24, and 48 h comparing cells inside and outside the TEM cell.|
At short time exposure (2-12 h) early activation of both p53-dependend and independend apoptotic pathways, and an induction of DNA breaks were observed.
In contrast, long time exposure (24-48 h) leads to the activation of pro-survival Ras pathways and an increased DNA synthesis.
These findings suggest that 900 MHz EMF exposure is capable to induce genotoxic damage and changes in gene expression.