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To study the influence of radiofrequency electromagnetic fields in vitro on the cell cycle, BrdU uptake and the induction of apoptosis and necrosis of human Mono Mac 6 cells.
Modulation type: pulsed
ばく露時間: continuous for 12 h
|ばく露時間||continuous for 12 h|
|チャンバの詳細||Two single-mode resonator cavities were placed inside a CO2 incubator that were randomly and blindly assigned for exposure [Schuderer et al., 2004].|
|ばく露装置の詳細||Six 35-mm Petri dishes were placed in each waveguide on a dish holder ensuring the correct placement in the H-field maxima of the standing waves.|
|Sham exposure||A sham exposure was conducted.|
|Additional information||Cells were treated simultaneously in the same incubator under the following conditions: untreated (sham or incubator control), RF exposed, sham exposed + PMA, RF exposed + PMA, sham exposed + gliotoxin, RF exposed + gliotoxin.|
No statistically significant differences in the induction of apoptosis or necrosis, cell cycle kinetics, or BrdU uptake were detected after radiofrequency electromagnetic field exposure. In the positive control cells treated with gliotoxin and PMA, a significant increase in apoptotic and necrotic cells was seen.
Cell cycle analysis or BrdU incorporation for 72 h after exposure showed no differences between radiofrequency electromagnetic field- or sham-exposed cells, whereas PMA treatment induced a significant accumulation of cells in G1 phase and a reduction in S-phase cells.