研究のタイプ: 医学/生物学の研究 (experimental study)

[ラジオ周波数照射されたヒトMono Mac 6細胞の細胞周期活性とアポトーシス、ネクローシスの比較研究] med./bio.

Comparative study of cell cycle kinetics and induction of apoptosis or necrosis after exposure of human mono mac 6 cells to radiofrequency radiation.

掲載誌: Radiat Res 2006; 166 (3): 539-543

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研究目的(著者による)

To study the influence of radiofrequency electromagnetic fields in vitro on the cell cycle, BrdU uptake and the induction of apoptosis and necrosis of human Mono Mac 6 cells.

詳細情報

The positive controls were with gliotoxin or tetradecanoylphorbol acetate (PMA) treated cells.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: pulsed
ばく露時間: continuous for 12 h
  • SAR: 2 W/kg unspecified

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 12 h
Modulation
Modulation type pulsed
Additional information

GSM-DTX mode, with transmission reduced to 12 active frames per 104 frames, leading to a crest factor of 69. The frame structure results in 2, 8, and 217 Hz components. [Lantow et al., 2006]

ばく露装置
ばく露の発生源/構造
チャンバの詳細 Two single-mode resonator cavities were placed inside a CO2 incubator that were randomly and blindly assigned for exposure [Schuderer et al., 2004].
ばく露装置の詳細 Six 35-mm Petri dishes were placed in each waveguide on a dish holder ensuring the correct placement in the H-field maxima of the standing waves.
Sham exposure A sham exposure was conducted.
Additional information Cells were treated simultaneously in the same incubator under the following conditions: untreated (sham or incubator control), RF exposed, sham exposed + PMA, RF exposed + PMA, sham exposed + gliotoxin, RF exposed + gliotoxin.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg unspecified 指定なし - -

Reference articles

  • Lantow M et al. (2006): [1800 MHzラジオ周波数照射後の2通りのヒト免疫細胞系のフリーラジカル放射とHSP70発現].
  • Schuderer J et al. (2004): [1800 MHzのインビトロ実験に用いる強力なばく露および環境制御機能を備えた高尖頭SARばく露装置]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露前
  • ばく露後

研究の主なアウトカム(著者による)

No statistically significant differences in the induction of apoptosis or necrosis, cell cycle kinetics, or BrdU uptake were detected after radiofrequency electromagnetic field exposure. In the positive control cells treated with gliotoxin and PMA, a significant increase in apoptotic and necrotic cells was seen.
Cell cycle analysis or BrdU incorporation for 72 h after exposure showed no differences between radiofrequency electromagnetic field- or sham-exposed cells, whereas PMA treatment induced a significant accumulation of cells in G1 phase and a reduction in S-phase cells.

研究の種別:

研究助成

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