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To study whether exposure to microwaves alters gene expression and cell viability in T-lymphoblastoid leukemia cells.
Four groups were examined: 1.) 2 hours sham exposure, 2.) 48 hours sham exposure, 3.) 2 hours exposure and 4.) 48 hours exposure.
literature reference: Stuchly, M. A., Stuchly, S. S. (1986). Experimental radio and microwave dosimetry. In: Polk, C., Postow, E. Handbook of Biological Effects of Electromagnetic Fields (pp. 229-272). Boca Raton, FL: CRC Press.
|ばく露時間||continuous for 2 or 48 hours|
|チャンバの詳細||aluminium box with 3 mm thickness in order to obtain 75 Ohm impedance; box contains a small aluminium septum, which divides the inner part into two equal parts; field was perpendicular to the septum plane; to allow gaseous exchange between incubator and petri dishes, one side of the TEM cell was made of a removable 2.5 mm mesh; five petri dishes of 10 cm diameter were placed in TEM cell at 1 cm distance above and below the septum|
|ばく露装置の詳細||transverse electromagnetic cell was placed in incubator|
|Sham exposure||A sham exposure was conducted.|
|Additional information||50 Hz-electric and magnetic field was generated by incubator, but did not exceed 0.8 V/m and 0.16 µT|
After 48 hours, a significantly decreased number of viable cells was found in the exposed cell cultures when compared to the 48 hours-control group. After 2 hours of exposure, the number of cells undergoing apoptosis was significantly increased in comparison to the 2 hours control group. This effect decreased after 48 hours, but remained statistically significant.
Analysis via microarray revealed that the gene expression of numerous genes involved in DNA repair, apoptosis, cell cycle regulation, angiogenesis and chromosomal organization was significantly down-regulated, respectively up-regulated in the exposed groups compared to the control groups. Most of the results were verified via Western blot.
The results indicate that microwave-exposure could induce changes in gene expression and influence the cell growth.