研究のタイプ: 医学／生物学の研究 (experimental study)

[マイクロ波電磁界（900 MHz）はT-リンパ芽球性白血病細胞株CCRF-CEMの遺伝子発現を調節する] med./bio.

Microwave electromagnetic field regulates gene expression in T-lymphoblastoid leukemia CCRF-CEM cell line exposed to 900 MHz

著者: Trivino Pardo JC, Grimaldi S, Taranta M, Naldi I, Cinti C
掲載誌: Electromagn Biol Med 2012; 31 (1): 1-18
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本研究では、高周波電磁界へのばく露が健康への悪影響を生じるかどうかを調べるため、TEMセルで発生させた900MHzマイクロ波電磁界への短期及び長期ばく露の存在下で、急性Tリンパ芽球性白血病細胞（CCRF-CEM）を培養した。遺伝子発現に対する高周波電磁界の影響を評価した結果、900MHzマイクロ波電磁界ばく露によって影響される機能的経路が明らかに示されたと述べている。

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研究目的（著者による）

To study whether exposure to microwaves alters gene expression and cell viability in T-lymphoblastoid leukemia cells.

詳細情報

Four groups were examined: 1.) 2 hours sham exposure, 2.) 48 hours sham exposure, 3.) 2 hours exposure and 4.) 48 hours exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: CW
ばく露時間: continuous for 2 or 48 hours

General information

literature reference: Stuchly, M. A., Stuchly, S. S. (1986). Experimental radio and microwave dosimetry. In: Polk, C., Postow, E. Handbook of Biological Effects of Electromagnetic Fields (pp. 229-272). Boca Raton, FL: CRC Press.

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 or 48 hours
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
チャンバの詳細 aluminium box with 3 mm thickness in order to obtain 75 Ohm impedance; box contains a small aluminium septum, which divides the inner part into two equal parts; field was perpendicular to the septum plane; to allow gaseous exchange between incubator and petri dishes, one side of the TEM cell was made of a removable 2.5 mm mesh; five petri dishes of 10 cm diameter were placed in TEM cell at 1 cm distance above and below the septum
ばく露装置の詳細 transverse electromagnetic cell was placed in incubator
Sham exposure A sham exposure was conducted.
Additional information 50 Hz-electric and magnetic field was generated by incubator, but did not exceed 0.8 V/m and 0.16 µT
パラメータ
測定量 値 種別 Method Mass 備考
SAR 3.5 mW/kg - - - -
電力密度 23.68 mW/m² - - - -
電界強度 3 V/m maximum 測定値 - -
磁界強度 8 mA/m - 測定値 - -

Reference articles

  • Marinelli F et al. (2004): [900 MHzの電磁界のT-リンパ芽球性白血病CCRF-CEM細胞へのばく露は、プロアポトーシスと生存促進シグナル間のアンバランスを誘発する]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

After 48 hours, a significantly decreased number of viable cells was found in the exposed cell cultures when compared to the 48 hours-control group. After 2 hours of exposure, the number of cells undergoing apoptosis was significantly increased in comparison to the 2 hours control group. This effect decreased after 48 hours, but remained statistically significant.
Analysis via microarray revealed that the gene expression of numerous genes involved in DNA repair, apoptosis, cell cycle regulation, angiogenesis and chromosomal organization was significantly down-regulated, respectively up-regulated in the exposed groups compared to the control groups. Most of the results were verified via Western blot.
The results indicate that microwave-exposure could induce changes in gene expression and influence the cell growth.

研究の種別:

研究助成

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