研究のタイプ: 医学/生物学の研究 (experimental study)

[ウキクサの成長とペロオキシダーゼ活性に対する400, 900, 1900 MHz電磁界の影響] med./bio.

Influence of 400, 900, and 1900 MHz electromagnetic fields on Lemna minor growth and peroxidase activity.

掲載誌: Bioelectromagnetics 2005; 26 (3): 185-193

この研究は、環境モニタリングのモデル植物として一般的に使用されているウキクサ(Lemna minor L.)を用いて、ギガヘルツTEM(GTEM)セル内で周波数400、900、1900 MHzの電界ばく露した後のウキクサの増殖およびペルオキシダーゼ活性を評価した。その結果、900 MHzの23 V / mの電界に2時間ばく露した場合、ウキクサの増殖は対照と比較して有意に減少した;400 MHzの同じ強度の電界ばく露した場合、そのような影響は見られなかった;900 MHzの変調波は増殖を強く阻害したが、400 MHzの変調波は増殖を有意には変化させなかった;どちらの周波数においても、ばく露時間が長くなるとほとんどの場合、増殖が減少し、最も強い電界強度(390 V / m)は増殖を強く阻害した;より低い電界強度(10 V / m)への14時間ばく露の場合、400および1900 MHzで有意な増殖低下が起きたが、900 MHzでは増殖への影響がなかった;ばく露されたウキクサのペルオキシダーゼ活性はばく露特性に応じて変化したが、その変化はほとんどの場合小さかった;例外は900 MHzの41 V / m電界への2時間ばく露の場合で、有意な増加(41%)が見られた、と報告している。

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研究目的(著者による)

Duckweed Lemna minor was used as a model plant for investigating the effects of electromagnetic fields on growth and changes in peroxidase activity.

詳細情報

Peroxidases are widely distributed enzymes in plants where they play many important roles in growth, differentiation and development processes. Different environmental factors can change peroxidase activity so it is often used as a biomarker for plants exposed to stress.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 400 MHz
Modulation type: CW
ばく露時間: continuous for 2, 4, and 14 h
ばく露2: 400 MHz
Modulation type: AM
ばく露時間: continuous for 2 h
ばく露3: 900 MHz
Modulation type: CW
ばく露時間: continuous for 2, 4, and 14 h
ばく露4: 900 MHz
Modulation type: AM
ばく露時間: continuous for 2 h
ばく露5: 1,900 MHz
Modulation type: CW
ばく露時間: continuous for 14 h

General information

Plants were exposed to 400 and 900 MHz at different field strengths (23, 41, and 390 V/m) for 2 h and to 400, 900, and 1900 MHz at 10 V/m for 14 h. At a field strength of 23 V/m, the effect of longer exposure (4 h) and field modulation (80% AM 1 kHz sinusoidal) was also tested.

ばく露1

主たる特性
周波数 400 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 2, 4, and 14 h
Additional information Reference article: Malaric K, Sarolic A, Roje V, Bartolic J, Modlic B. 2001. Measured distribution of electric field in GTEM-cell. In: Proc. IEEE International Symposium on Electromagnetic Compatibility, Vol. 1., Montreal, Canada, August, 2001, pp 139-141.
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • GTEMセル
チャンバの詳細 The GTEM cell was 0.6 m wide and 0.4 m high at the end, and 1.15 m long. The inner conductor was at 3/4 height with an angle septum/bottom plate of 15°, and septum/top plate of 5°.
ばく露装置の詳細 Plants were kept in plastic Petri dishes (d = 9 cm, h = 1.5 cm) on the surface of a solid nutrient medium layer that was 0.2 cm thick. Five Petri dishes were arranged like the Olympic rings, in the same plane and on a dielectric support, perpendicular to the electric field, and placed below the septum for the three lower field strengths but on the septum for the highest field strength. In both cases, the area where plants were placed had the most uniform field distribution (± 0.1 dB).
Sham exposure A sham exposure was conducted.
Additional information For each experiment, control plants in field free environment were handled in the same way and kept in the same growth conditions as treated ones. In preliminary experiments, no significant differences between plants sham exposed in the GTEM cell and plants outside the GTEM cell were found. The temperature at the beginning and at the end of the measurements as well as between control and exposed plants varied no more than ± 0.1 °C.
パラメータ
測定量 種別 Method Mass 備考
電界強度 10 V/m unspecified 測定値および計算値 - for 14 h
電界強度 23 V/m unspecified 測定値および計算値 - for 2 h and 4 h
電界強度 41 V/m unspecified 測定値および計算値 - for 2 h
電界強度 390 V/m unspecified 測定値および計算値 - for 2 h

ばく露2

主たる特性
周波数 400 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 2 h
Modulation
Modulation type AM
Modulation frequency 1 kHz
Modulation depth 80 %
Additional information

sinusoidal

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電界強度 23 V/m unspecified 測定値および計算値 - -

ばく露3

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 2, 4, and 14 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電界強度 10 V/m unspecified 測定値および計算値 - for 14 h
電界強度 23 V/m unspecified 測定値および計算値 - for 2 h and 4 h
電界強度 41 V/m unspecified 測定値および計算値 - for 2 h
電界強度 390 V/m unspecified 測定値および計算値 - for 2 h

ばく露4

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 2 h
Modulation
Modulation type AM
Modulation frequency 1 kHz
Modulation depth 80 %
Additional information

sinusoidal

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電界強度 23 V/m unspecified 測定値および計算値 - -

ばく露5

主たる特性
周波数 1,900 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 14 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
電界強度 10 V/m unspecified 測定値および計算値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The growth of plants exposed to the 23 V/m electric field of 900 MHz for 2 h significantly decreased in comparison with the control. An electric field of the same electric field strength but at 400 MHz did not have such effect. A modulated field at 900 MHz inhibited the growth, while at 400 MHz modulation did not influence the growth significantly. At both frequencies a longer irradiation mostly decreased the growth and the highest electric field (390 V/m) strongly inhibited the growth. Irradiation of plants to lower field strength (10 V/m) for 14 h caused significant decrease at 400 and 1900 MHz, while 900 MHz did not influence the growth.
Peroxidase activity in irradiated plants varied, depending on the exposure characteristics. Revealed alterations were mostly small, except in plants exposed for 2 h to 41 V/m at 900 MHz where a significant increase was found.
The data suggest that investigated electromagnetic fields might influence plant growth and, to some extent, peroxidase activity. However, the effects strongly depended on the field characteristics.

研究の種別:

研究助成

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