この研究は、特別設計した固定モード反響チャンバー内でトマトの植物体に短時間低レベル電磁界ばく露（900MHz、5 V/ m、10分間）を行い、ばく終了直後にmRNAの変化を調べた。その結果、ストレス関連のmRNA（カルモジュリン、カルシウム依存性プロテインキナーゼ、プロテイナーゼ阻害因子）が環境ストレス反応に典型的な様式で、大量に、急速に蓄積した；これら転写物のポリソームRNAへの蓄積も生じたが、時間的に遅れた（すなわち、コードされたタンパク質は翻訳されたが、新しく合成されたmRNAは直ちにはポリソームに動員されなかったことを意味する）；転写物の蓄積は通常のカルシウムイオンレベルにおいて最大となり、カルシウムイオンレベル上昇により抑制された、などを報告している。
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To measure rapid molecular responses (i.e. accumulation of stress-related transcripts and cellular energy state) in tomato plants after short-term exposure to radiofrequency electromagnetic fields.
All of the transcripts investigated are wound-up-regulated. Thus, similarities between wounding and radiofrequency electromagnetic field exposure could be studied. Additionally, since two of them encode calcium-binding proteins, the authors examined the effects of varying calcium availability to determine if there was any feedback mechanism involved in their transcription.
ATP concentration and adenylate energy charge were also measured after exposure, as well as the effect of an uncoupling agent on transcript accumulation in order to assess the influence of radiofrequency exposure on cellular energy status and to correlate this with changes in mRNA abundances.
ばく露時間: continuous for 10 min
|ばく露時間||continuous for 10 min|
|チャンバの詳細||Seeds were grown inside two identical custom-made (plywood) culture chambers with a hydroponic system. One of the chambers was armoured with a multi-layered aluminum material to protect the plants from exposure.|
|ばく露装置の詳細||When the plants were 3 weeks old, the whole culture chamber was placed in the MSRC (24 h prior to irradiation) in a volume where the field was homogeneous and isotropic. The MSRC (mode stirred reverberation chamber) was a large (200 m³) Faraday cage with an RF-EMF that irradiated plants from several directions with different polarizations (due to reflection properties of the MSRC).|
The data provide new evidence supporting the hypothesis that plants perceive and respond to microwave exposure as though it was an injurious treatment. In addition to the rapidity of the response (stress-related mRNA accumulation) and its dependency on the second messenger calcium, the authors observed a strong correlation between all the parameters measured (total and polysomal transcript abundance, ATP concentration and adenylate energy charge.