研究のタイプ: 医学/生物学の研究 (experimental study)

[高周波(900 MHz)低振幅電磁界:トマトの転写、翻訳、カルシウムとエネルギーの補給を変化させる真の環境刺激] med./bio.

High frequency (900 MHz) low amplitude (5 V m-1) electromagnetic field: a genuine environmental stimulus that affects transcription, translation, calcium and energy charge in tomato.

掲載誌: Planta 2008; 227 (4): 883-891

この研究は、特別設計した固定モード反響チャンバー内でトマトの植物体に短時間低レベル電磁界ばく露(900MHz、5 V/ m、10分間)を行い、ばく終了直後にmRNAの変化を調べた。その結果、ストレス関連のmRNA(カルモジュリンカルシウム依存性プロテインキナーゼ、プロテイナーゼ阻害因子)が環境ストレス反応に典型的な様式で、大量に、急速に蓄積した;これら転写物のポリソームRNAへの蓄積も生じたが、時間的に遅れた(すなわち、コードされたタンパク質翻訳されたが、新しく合成されたmRNAは直ちにはポリソームに動員されなかったことを意味する);転写物の蓄積は通常のカルシウムイオンレベルにおいて最大となり、カルシウムイオンレベル上昇により抑制された、などを報告している。

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研究目的(著者による)

To measure rapid molecular responses (i.e. accumulation of stress-related transcripts and cellular energy state) in tomato plants after short-term exposure to radiofrequency electromagnetic fields.

詳細情報

All of the transcripts investigated are wound-up-regulated. Thus, similarities between wounding and radiofrequency electromagnetic field exposure could be studied. Additionally, since two of them encode calcium-binding proteins, the authors examined the effects of varying calcium availability to determine if there was any feedback mechanism involved in their transcription.
ATP concentration and adenylate energy charge were also measured after exposure, as well as the effect of an uncoupling agent on transcript accumulation in order to assess the influence of radiofrequency exposure on cellular energy status and to correlate this with changes in mRNA abundances.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
ばく露時間: continuous for 10 min

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 10 min
ばく露装置
ばく露の発生源/構造
  • MSRC
チャンバの詳細 Seeds were grown inside two identical custom-made (plywood) culture chambers with a hydroponic system. One of the chambers was armoured with a multi-layered aluminum material to protect the plants from exposure.
ばく露装置の詳細 When the plants were 3 weeks old, the whole culture chamber was placed in the MSRC (24 h prior to irradiation) in a volume where the field was homogeneous and isotropic. The MSRC (mode stirred reverberation chamber) was a large (200 m³) Faraday cage with an RF-EMF that irradiated plants from several directions with different polarizations (due to reflection properties of the MSRC).
パラメータ
測定量 種別 Method Mass 備考
電界強度 5 V/m - - - -

Reference articles

  • Beaubois E et al. (2007): [植物における細胞内情報伝達:トマトにおいて電磁界刺激で生じる2つの急速に植物体に伝達される信号の証拠]
  • Roux D et al. (2006): [電磁界(900 MHz)はトマトに一定の分子反応をもたらす]
  • Vian A et al. (2006): [マイクロ波照射は植物の遺伝子発現に影響する]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The data provide new evidence supporting the hypothesis that plants perceive and respond to microwave exposure as though it was an injurious treatment. In addition to the rapidity of the response (stress-related mRNA accumulation) and its dependency on the second messenger calcium, the authors observed a strong correlation between all the parameters measured (total and polysomal transcript abundance, ATP concentration and adenylate energy charge.

研究の種別:

研究助成

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