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To study the effect of mobile phone intensity microwave irradiation on tomato plant studying the accumulation of a stress-related transcript that responds very rapidly to even small environmental stimulations.
Leaf tissue was collected at various times (0, 5, 15, 30, 60 min.) after the end of exposure.
|ばく露時間||continuous for 10 min|
|チャンバの詳細||The MSRC (mode stirred reverberation chamber) consisted of a large room (200 m³) with a double-layered metal wall, acting as a Faraday cage, a log-periodic antenna, and a rotary stirrer creating different patterns of multiple reflections on the chamber walls, thus randomizing the polarization of the electromagnetic waves. Tomato plants were grown inside EMF-permeable culture chambers. Shielded chambers were enclosed in a polymer mesh covered with an aluminium layer causing 45 dB signal attenuation to protect the plants from exposure.|
|ばく露装置の詳細||When the plants were 3 weeks old, the whole culture chamber was placed in the MSRC (18 h prior to irradiation) in a defined working volume where the field was statistically isotropic and homogeneous.|
|Additional information||The fourth terminal leaf (exposed and shielded) was collected at various times after exposure (0, 5, 15, 30, and 60 min). Control plants were collected before stimulation.|
The data showed that low amplitude, short duration, 900 MHz electromagnetic field evoked the accumulation of the stress-related transcript (mRNA). This accumulation was rapid (peaking 5-15 min after exposure), strong (3.5-fold), and was similar to that evoked by injurious stimuli.
In some experiments, the accumulation began immediately after the end of the exposure, while in others there was a short delay. In all cases, the response was maximal at 5-15 min. after the end of exposure, in some cases declined somewhat at 30 min., but generally remained at high levels until 60 min.