研究のタイプ: 医学/生物学の研究 (experimental study)

[低電力マイクロ波放射はP27Kip1発現をアップレギュレートすることによって、ウサギ水晶体上皮細胞の増殖を阻害する] med./bio.

Low power microwave radiation inhibits the proliferation of rabbit lens epithelial cells by upregulating P27Kip1 expression.

掲載誌: Mol Vis 2004; 10: 138-143

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研究目的(著者による)

To study the effects of low power microwave irradiation on the proliferation of cultured rabbit eye lens epithelial cells.

詳細情報

There was less than a 0.6 °C difference between the temperature of the culture medium in the exposed and sham-exposed cells (after 8 h of irradiation).
Cell proliferation through the cell cycle are regulated by the sequential activity of various cyclin-dependent kinases. In addition, cyclin-dependent kinases activity can be negatively regulated by a group of proteins termed cyclin-dependent kinases inhibitors. One family of cyclin-dependent kinases inhibitors includes P21WAF1 and P27Kip1. Overexpression of these inhibitors can attenuate the proliferative response, whereas a reduction in their expression increases proliferation.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2,450 MHz
Modulation type: CW
ばく露時間: continuous for 8 h
ばく露2: 2,450 MHz
Modulation type: CW
ばく露時間: continuous for 4, 6, and 8 h

ばく露1

主たる特性
周波数 2,450 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 8 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • 詳細不明
ばく露装置の詳細 Cells were exposed under controlled temperature conditions (25 °C) in 35 mm² culture dishes or in 96-well culture plates.
Sham exposure A sham exposure was conducted.
Additional information Sham exposed cells were treated in the same way as the exposed cells except that the power to the antenna was not activated.
パラメータ
測定量 種別 Method Mass 備考
電力密度 0.1 mW/cm² - 測定値 - -
電力密度 0.25 mW/cm² - 測定値 - -
電力密度 0.5 mW/cm² - 測定値 - -
電力密度 1 mW/cm² - 測定値 - -
電力密度 2 mW/cm² - 測定値 - -

ばく露2

主たる特性
周波数 2,450 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 4, 6, and 8 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力密度 2 mW/cm² - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

After 8 h of irradiation, cells exposed to 0.50, 1.00, and 2.00 mW/cm² exhibited decreased cell viability, increased cell condensation and an inhibition of DNA synthesis. The cells showed significant G0/G1 arrest in the cell cycle. No obvious changes could be detected in the groups exposed to 0.10 and 0.25 mW/cm² microwave irradiation.
Protein expression of P27Kip1 was markedly increased after exposure. However, the mRNA levels were unchanged. There were no detectable differences in P21WAF1 protein expression and mRNA levels between microwave exposed and control groups.
This study indicates that low power microwave irradiation higher than 0.50 mW/cm² can inhibit eye lens epithelial cell proliferation, and increase the expression of P27Kip1.

研究の種別:

研究助成

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