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To study the effects of low power microwave irradiation on the proliferation of cultured rabbit eye lens epithelial cells.
There was less than a 0.6 °C difference between the temperature of the culture medium in the exposed and sham-exposed cells (after 8 h of irradiation). Cell proliferation through the cell cycle are regulated by the sequential activity of various cyclin-dependent kinases. In addition, cyclin-dependent kinases activity can be negatively regulated by a group of proteins termed cyclin-dependent kinases inhibitors. One family of cyclin-dependent kinases inhibitors includes P21WAF1 and P27Kip1. Overexpression of these inhibitors can attenuate the proliferative response, whereas a reduction in their expression increases proliferation.
After 8 h of irradiation, cells exposed to 0.50, 1.00, and 2.00 mW/cm² exhibited decreased cell viability, increased cell condensation and an inhibition of DNA synthesis. The cells showed significant G0/G1 arrest in the cell cycle. No obvious changes could be detected in the groups exposed to 0.10 and 0.25 mW/cm² microwave irradiation. Protein expression of P27Kip1 was markedly increased after exposure. However, the mRNA levels were unchanged. There were no detectable differences in P21WAF1 protein expression and mRNA levels between microwave exposed and control groups. This study indicates that low power microwave irradiation higher than 0.50 mW/cm² can inhibit eye lens epithelial cell proliferation, and increase the expression of P27Kip1.