この研究は、低電力密度のマイクロ波放射がウサギの水晶体上皮細胞（LEC）においてギャップ結合細胞間コミュニケーション（GJIC）の変化を引き起こすか否かを調べた。ウサギの眼に電力密度5 mW / cm2または10 mW / cm2のマイクロ波の3時間ばく露を与えた。光退色後蛍光回復（FRAP）法を使用して、GJICを測定した。LECにおけるコネキシン43の局在および機能は、レーザー走査型共焦点顕微鏡法で測定した。その結果、ウサギLECのGJICはマイクロ波ばく露によって抑制され、それは特に10 mW / cm（2）ばく露群で顕著であった；5 mW / cm（2）ばく露を3時間受けたLECにおいて、コネキシン43陽性染色は減少した；10 mW / cm（2）ばく露群では、細胞内空間の蓄積および細胞質の内在化が明確に検出された、と報告している。
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To study the changes in gap junctional intercellular communication mediated by low power density microwave exposure in rabbits eye lens epithelial cells and its mechanisms.
16 rabbits were investigated: 8 rabbits were used for investigation of gap junctional intercellular communication function (4 rabbits received 5 mW/cm², 4 rabbits received 10 mW/cm²). The other 8 rabbits were used for detection of localization and function of connexin 43. One eye served as experimental eye while the other eye served as control.
|ばく露時間||continuous for 3 h|
|Distance between exposed object and exposure source||2 m|
|ばく露装置の詳細||The head of the rabbit was fixed at the distance of 2 m from the emission machine, and one open eye was exposed.|
|Additional information||The other eye served as control and was covered tightly by copper grid cloth shielding material.|
The gap junctional intercellular communication of eye lens epithelial cells was inhibited by microwave irradiation especially in the 10 mW/cm² exposed samples. A decrease in connexin 43-positive staining was found in 5 mW/cm² treated eye lens epithelial cells.
In conclusion, low power densities microwave irradiation induces damage to connexin 43 and inhibits the gap junctional intercellular communication of eye lens epithelial cells. These changes result in an osmotic imbalance within the eye lens and induce early cataract.