研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒトレンズ細胞のDNA損傷と熱ショックタンパク70の発現に対する1.8 GHzラジオ周波数電磁界の影響] med./bio.

Effects of 1.8 GHz radiofrequency field on DNA damage and expression of heat shock protein 70 in human lens epithelial cells.

掲載誌: Mutation Research - Fundamental and Molecular Mechanism of Mutagenesis 2006; 602 (1-2): 135-142

この研究は、GSM携帯電話の1.8 GHz無線周波電磁界RFばく露を受けたヒト水晶体上皮細胞(hLEC)でのDNA損傷熱ショックタンパク質70(Hsp70)の発現および細胞増殖への影響を調べた。Xc-1800 RFばく露システムを使用して、1.8 GHz(217 Hzの振幅変調)のGSM信号を、比吸収率SAR)1、2、3 W / kgで細胞に与えた。RFへの2時間ばく露終了から0、30、60、120、240分間培養したhLECでのDNA損傷をコメットアッセイによって調べた。ウエスタンブロットとRT-PCRを使用して、RFばく露後のhLECにおけるHsp70の発現を測定した。細胞の増殖速度は、ばく露後0、1、4日目にブロモデオキシウリジン取り込みにより評価した。その結果、1および2 W / kgのばく露の場合、ばく露群と擬似ばく露群(対照群)の間のDNA切断の差は、どの培養時間でも有意ではなかった; 3 W / kgの場合、DNA損傷は、ばく露後0および30分の培養時間で有意に増加したが、60、120、または240分の培養時間ではそうではなかった;Hsp70のmRNAおよびタンパク質発現がすべてのばく露群で見られた;2および3 W / kgの場合、Hsp70タンパク質発現の増加は有意であったが、Hsp70 mRNA発現の変化は有意でなかった;いずれのばく露群においても、擬似ばく露群と比べ、細胞増殖速度に差は見られなかった、と報告している。

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研究目的(著者による)

To investigate the in vitro effects of 1.8 GHz radiofrequency field (GSM) on human eye lens cells.

詳細情報

As the specific absorption rate of 4 W/kg is the accepted dose threshold for thermal and non-thermal effects, eye lens cells were exposed to three different specific absorption rates lower than this threshold (1, 2 and 3 W/kg) to avoid thermal effects.
DNA damage and Hsp expression were explored 0, 0.5, 1, 2, and 4 hours after exposure. Cell proliferation was determined on days 0, 1 and 4 after exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1.8 GHz
Modulation type: pulsed
ばく露時間: continuous for 2 h

ばく露1

主たる特性
周波数 1.8 GHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 2 h
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

There are contradictory statements in the article, whether the signal was 217 Hz GSM or CW.

ばく露装置
ばく露の発生源/構造
チャンバの詳細 Two rectangular anechoic single-mode resonator cavities with fans, one for RF and one for sham exposure, were placed in an incubator at 37 °C, 5% CO2, saturated vapour.
ばく露装置の詳細 Six 35-mm Petri dishes containing 2 ml of medium were placed on dish holders inside each waveguide exactly in the H-field maximum of the standing wave.
Sham exposure A sham exposure was conducted.
Additional information Negative and positive (40 °C for 0.5 h) incubator controls were also used.
パラメータ
測定量 種別 Method Mass 備考
SAR 1 W/kg - - - -
SAR 2 W/kg - - - -
SAR 3 W/kg - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The results showed no significant difference of DNA damage between the exposed and sham-exposed groups induced by specific absorption rates of 1 and 2 W/kg at any incubation time point. The DNA damage caused by 3 W/kg irradiation was significantly increased at the times of 0 and 30 minutes after exposure, a phenomenon that could not be seen at the time points of 1, 2, and 4 hours.
Hsp70 mRNA expression as well as Hsp70 protein expression was found in all exposure groups. Exposure at specific absorption rates of 2 and 3 W/kg for 2 hours exhibited significantly increased Hsp70 protein expression, while no change in Hsp70 mRNA expression could be found in any of the groups.
No difference of the cell proliferation rate between the sham-exposed and exposed cells was found.

研究の種別:

研究助成

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