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To investigate the in vitro effects of 1.8 GHz radiofrequency field (GSM) on human eye lens cells.
As the specific absorption rate of 4 W/kg is the accepted dose threshold for thermal and non-thermal effects, eye lens cells were exposed to three different specific absorption rates lower than this threshold (1, 2 and 3 W/kg) to avoid thermal effects.
DNA damage and Hsp expression were explored 0, 0.5, 1, 2, and 4 hours after exposure. Cell proliferation was determined on days 0, 1 and 4 after exposure.
|ばく露時間||continuous for 2 h|
|チャンバの詳細||Two rectangular anechoic single-mode resonator cavities with fans, one for RF and one for sham exposure, were placed in an incubator at 37 °C, 5% CO2, saturated vapour.|
|ばく露装置の詳細||Six 35-mm Petri dishes containing 2 ml of medium were placed on dish holders inside each waveguide exactly in the H-field maximum of the standing wave.|
|Sham exposure||A sham exposure was conducted.|
|Additional information||Negative and positive (40 °C for 0.5 h) incubator controls were also used.|
The results showed no significant difference of DNA damage between the exposed and sham-exposed groups induced by specific absorption rates of 1 and 2 W/kg at any incubation time point. The DNA damage caused by 3 W/kg irradiation was significantly increased at the times of 0 and 30 minutes after exposure, a phenomenon that could not be seen at the time points of 1, 2, and 4 hours.
Hsp70 mRNA expression as well as Hsp70 protein expression was found in all exposure groups. Exposure at specific absorption rates of 2 and 3 W/kg for 2 hours exhibited significantly increased Hsp70 protein expression, while no change in Hsp70 mRNA expression could be found in any of the groups.
No difference of the cell proliferation rate between the sham-exposed and exposed cells was found.