研究のタイプ: 医学/生物学の研究 (experimental study)

[ラット脳の神経膠に対する900 MHz GSM急性ばく露の影響 -時間依存性研究ー] med./bio.

Effect of an acute 900MHz GSM exposure on glia in the rat brain: a time-dependent study.

掲載誌: Toxicology 2007; 238 (1): 23-33

携帯電話使用の増加により、人の脳に悪影響をもたらす無線周波数電磁場の潜在的リスクを評価する必要がある。この研究で我々は、ラットの脳にGSMばく露(15min、脳平均SAR=6W/kg、900MHz信号)を1回行い、2日、3日、6日、10日後のグリア細胞変化を評価するため、GFAP(glial fibrillary acidic protein=グリア細胞繊維性酸性タンパク質)を測定した。GFAPの染まった表面部分の統計的に有意な増加は、前頭皮質尾状核被殻におけるばく露の2日後に確認された。同様に統計的に有意な増加は、同部分小脳皮質におけるばく露の2日後に確認された。我々の結果は、Mausset-Bonnefont他の研究[Mausset-Bonnefont A.L、Hirbec H、Bonnefront X、Privat A、Vignon J、de Seze R、2004、Neurobiol.Dis.17、445-454]を裏付け、6W/kgの脳平均SARにおける15minGSM急性ばく露後のグリア細胞反応の発現を示した。この影響が有害であるかどうかは今後の研究に委ねられる。

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研究目的(著者による)

This in vivo study was performed to reproduce and complete the result of publication 6611 which found an astrocytes activation (glial reaction) in the rat brain after a single 15 min GSM exposure.

詳細情報

Several brain regions (frontal cortex, striatum, globus pallidus, hippocampus, cortex of cerebellum) were examined in their glial fibrillary acidic protein (GFAP) level as it is a well-established marker to study astrocytes activation.
48 male rats (six weeks old, weighting 220-250 g) were divided into four exposure groups nine animals each and two sham exposure groups six animals each.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 15 min
  • SAR: 6 W/kg average over mass (brain) (±20%)

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 15 min
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Additional information

GSM

ばく露装置
ばく露の発生源/構造
チャンバの詳細 The exposure setup has been described in [Dubreuil et al., 2002; Mausset et al., 2001]. Rats were restrained in individual 5-mm thick perforated Plexiglas tubes, 6 cm in diameter and 15 cm long, with an open-ended cone (3 cm long) in which the rat head was inserted. Animals could rotate their heads but its longitudinal position was fixed.
ばく露装置の詳細 An RF generator was connected to a four-output divider, allowing simultaneous exposure of four animals. Each output was connected to a loop antenna that was placed against the cone directly above the rat's head for local exposure. A Luxtron optical temperature probe was fixed on the skin of the rat's head below the antenna.
Sham exposure A sham exposure was conducted.
Additional information RF and sham exposed animals were placed at the same time in separate anechoic chambers. Two additional control groups were included: a negative cage control group without any manipulation and an immunostaining positive control group, 4 h after an i.v. injection of LipoPolySaccharide (LPS) solution in saline.
パラメータ
測定量 種別 Method Mass 備考
SAR 6 W/kg average over mass 計算値 brain ±20%

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Two days after the 900 MHz GSM exposure a statistically significant increase of GFAP stained area was observed in the frontal cortex and the striatum. A smaller statistically significant increase was found three days after exposure in the same areas and in the cerebellum cortex. These increased GFAP levels indicating astrocytes activation were transient.
The data confirm the result of publication 6611 concerning glial reaction in specific brain regions. Whether this effect could be harmful should be investigated in further studies.

研究の種別:

研究助成

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