研究のタイプ: 医学/生物学の研究 (experimental study)

[無線周波ばく露後のマウス海馬でのカルシウム結合タンパク質の免疫活性変化に対するチョウセンニンジンの神経保護作用] med./bio.

Neuroprotective effect of ginseng against alteration of calcium binding proteins immunoreactivity in the mice hippocampus after radiofrequency exposure.

掲載誌: Biomed Res Int 2013: 812641

この研究は、無線周波RFばく露によるマウス海馬でのカルシウム結合タンパク質(CaBPs)の免疫活性の変化の有無、およびその変化に対する紅チョウセンニンジン抽出液(RG)の神経保護作用を調べた。6週齢ICR雄マウス30匹を、10匹ずつの3群に分けた:擬似ばく露群、RFばく露群(ホーンアンテナによる835MHz全身ばく露SAR 1.6W/kg)、RF+RG同時ばく露群。ばく露は、1日1時間、30日連続して行い、ばく露終了後、脳の海馬組織検査を行った。その結果、RF群では、アンモン角でCaBPsをもつ樹状突起分岐が失われ、歯状回顆粒細胞免疫組織化学的染色の強度の低下が見られたが、RF+RG群では何も見られなかった;免疫活性密度の平均値は、RF群では対照群との有意差があったが、RF+RG群では有意な差はなかった、と報告している。

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研究目的(著者による)

To examine the potential protective effect of ginseng on the hippocampus of mice exposed to a radiofrequency electromagnetic field.

詳細情報

Three groups of mice were examined (n=10 per group): 1.) sham exposure, 2.) exposure and 3.) exposure + 30mg/kg ginseng extract.
Calcium binding proteins like calbindin D28k, parvalbumin and calretinin, play an important role in maintaining the intracellular calcium homeostasis. These proteins are specifically distributed in the central nervous system. Ginseng is a widely used herbal medicine with a possibly protective effect on the central nervous system. Therefore, the immunoreactivity of calcium binding proteins in the hippocampus of mice was examined with and without an administration of ginseng extract.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 835 MHz
ばく露時間: 5 h/day for 30 consecutive days

ばく露1

主たる特性
周波数 835 MHz
タイプ
  • electromagnetic field
ばく露時間 5 h/day for 30 consecutive days
ばく露装置
ばく露の発生源/構造
チャンバの詳細 mice were exposed in a standard mouse cage of 22 inches (remark EMF-Portal: ca. 56 cm)
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 1.6 W/kg mean - - -
電界強度 59.56 V/m - 計算値 - -
電力 2.5 W - 備考を参照のこと。 - output power, simulated

Reference articles

  • Maskey D et al. (2010): [マウス海馬への835 MHz無線周波の慢性ばく露はカルビジンとGFAPの免疫反応分布を変化させる]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Significant decreases in the intensity of the immunoreactivities regarding calbindin D28k, parvalbumin and calretinin were observed in the exposure group without ginseng when compared to the control group in most of the hippocampal areas. However, no significant differences were observed between the exposure group with ginseng administration and the control group.
Additionally, histopathological changes occurred: In the hippocampus of exposed mice without ginseng, the dendritic arborization decreased compared to the control group, but not in exposed mice with ginseng administration.
The authors conclude that a radiofrequency electromagnetic field could disturb the calcium homeostasis via changing the immunoreactivity of calcium binding proteins in the hippocampus of mice and that ginseng could have a protective effect.

研究の種別:

研究助成

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