研究のタイプ: 医学/生物学の研究 (experimental study)

[携帯電話の無線周波数電磁界にばく露したマウスの脳における水チャネルタンパク質、アクアポリン-4の発現] med./bio.

Expression of the water channel protein, aquaporin-4, in mouse brains exposed to mobile telephone radiofrequency fields.

掲載誌: Pathology 2009; 41 (5): 473-475

この論文は、携帯電話RF電磁界の急性または慢性ばく露により、水チャネルタンパク質であるアクアポリン-4(AQP-4)の発現を上方調節するか否かを調べた。マウスを対象に、急性ばく露実験では900MHzの遠方電磁界へのSAR 4 W/kg の60分間全身ばく露を1回、長期ばく露実験では同様の60分間ばく露を連続5日/週で104週間与えた。対照としては「擬似ばく露群」の他に、ばく露ジュールによる拘束ストレスの対照としての「ケージ内自由行動群」、AQP-4を上方調節する薬剤を投与した「ポジティブ・コントロール群」を用いた。両方のばく露後における脳切片検査の結果、「擬似ばく露群」、「ケージ内自由行動群」に比較して「ばく露群」の脳におけるAQP-4発現の増加はなかった。このことは、血液脳関門の透過性に顕著な増加はないことを示唆している。

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研究目的(著者による)

To study whether acute (60 minutes) or long-term (two years) exposure to mobile phone radiofrequency fields produces up-regulation of the water channel protein, aquaporin-4.

詳細情報

Aquaporins are a family of small hydrophobic membrane proteins that serve as a selective pore through which water traverses the plasma membrane of different cell types. Aquaporin-4 is up-regulated in cerebral oedema of vasogenic type where there is increased blood-brain barrier permeability. The authors immunostained brains for aquaporin-4, which had previously been used in vascular tracer studies with albumin (Finnie et al. 2001, Finnie et al. 2002, and Finnie and Blumbergs 2004).
10 mice were acutely exposed, 10 mice were long-term exposed, 20 mice were sham-exposed and 20 mice were cage control animals (10 for each exposure condition). A positive control group was given a clostridial toxin known to cause microvascular endothelial injury, severe vasogenic oedema and up-regulation of aquaporin-4.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 60 min
acute exposure
ばく露2: 900 MHz
Modulation type: pulsed
ばく露時間: continuous on 5 days/week for 104 weeks
long time exposure

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • far field
ばく露時間 continuous for 60 min
Additional information acute exposure
Modulation
Modulation type pulsed
Pulse width 0.6 ms
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 mice in perpex tubes arrangd radially around the dipole antenna
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 4 W/kg - - whole body -

ばく露2

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • far field
ばく露時間 continuous on 5 days/week for 104 weeks
Additional information long time exposure
Modulation
Modulation type pulsed
Pulse width 0.6 ms
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
SAR 4 W/kg - - whole body -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

There was no increase in aquaporin-4 expression in mouse brains exposed to mobile phone microwaves compared to controls (sham exposure and cage control) brains after short or long-term exposure, while aquaporin-4 was substantially upregulated in the brains of mice given the clostridial toxin.
The authors conclude that brains exposed to mobile phone radiofrequency fields for a short or long duration did not show any immunohistochemically detectable up-regulation of the water channel protein, aquaporin-4, suggesting that there was no significant increase in blood-brain barrier permeability.

研究の種別:

研究助成

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