研究のタイプ: 医学/生物学の研究 (experimental study)

[50 Hz磁界とUV照射にばく露されたマウスの表皮でのODCとポリアミン] med./bio.

Epidermal ornithine decarboxylase and polyamines in mice exposed to 50 Hz magnetic fields and UV radiation.

掲載誌: Bioelectromagnetics 1998; 19 (6): 388-391

この研究は、マウス上皮におけるオルニチンデカルボキシラーゼODC)およびポリアミンに対する磁界MF)および模擬的太陽放射(SSR)の影響を調べた。その結果、慢性ばく露においては、SSR単独ばく露群との比較において、MFとSSRの組み合わせばく露群では、ODC活性またはポリアミンに対する持続的影響を引き起こさなかった;急性24時間ばく露においては、100 μTのMFばく露群で、プトレシン上昇およびODC活性低下が観察された;SSR急性24時間ばく露では、最小紅斑ドース(MED)の2倍のレベルでの単回ばく露で、影響は見られなかった、と報告している。

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研究目的(著者による)

The effects of an acute and chronic exposure of mice to a 50 Hz magnetic field on the epidermal level of polyamines and ornithine decarboxylase enzyme activity should be investigated.

詳細情報

The level of polyamines is increased in cancerous tissues and ornithine decarboxylase generates polyamines, so these parameters were used as markers for skin tumorigenesis. Tumor data of the same study was published elsewhere (Kumlin et al. 1998).
Transgenic K2 mice, which show a 30-50 fold higher ornithine decarboxylase enzyme activity compared to wild type mice, were used together with wild type mice.
For acute exposure experiments, the following groups with wild type and transgenic mice each were used (n=6 each): 1) exposure to UV radiation, 2) exposure to the magnetic field, 3) sham exposure. For chronic exposure experiments, further groups with wild type and transgenic mice each were used (n=10 each): 4) exposure to UV radiation, 5) exposure to UV radiation and continuous magnetic field , 6) exposure to UV and intermittent magnetic field and 7) sham exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 24 h
acute exposure
ばく露2: 50 Hz
ばく露時間: continuous for 10.5 months
continous chronic exposure
  • not stated
ばく露3: 50 Hz
ばく露時間: intermittend for 10.5 months
intermittend chronic exposure with various MF intensities

General information

UV exposure groups received a single dose of 400 J/m² during acute exposure (group 1) and 200 J/m² for 35 min three times per week, respectively, during chronic exposure conditions (groups 4-6)

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 24 h
Additional information acute exposure
ばく露装置
ばく露の発生源/構造
  • 詳細不明
チャンバの詳細 polycarbonate cages
ばく露装置の詳細 temperature and relative humidity were 23 ± 1°C and 50 ± 15%, respectively
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 100 µT - - - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 10.5 months
Additional information continous chronic exposure
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
Additional information 50 Hz MF exposure of the sham-exposed group was less than 0.05 µT
パラメータ
測定量 種別 Method Mass 備考
磁束密度 - - - - not stated

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 intermittend for 10.5 months
Additional information intermittend chronic exposure with various MF intensities
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.3 µT minimum - - -
磁束密度 13 µT - - - -
磁束密度 130 µT maximum - - -

Reference articles

  • Kumlin T et al. (1998): [ODCトランスジェニックと非トランスジェニックマウスでのUV-誘発皮膚腫瘍形成に対する50 Hz磁界の影響]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In the acute exposure, a significant increase of the putrescine and spermidine levels was observed in transgenic mice exposed to the magnetic field (group 2) compared to sham exposed mice. In the same group, a significant decrease of the ornithine decarboxylase enzyme activity was spotted in transgenic mice compared to sham exposed mice. In wild type mice, no significant differences were found between the groups.
In wild type mice chronically exposed to UV radiation (group 4), a significant increase of the spermidine and spermine levels and a significant decrease of the putrescine level was observed compared to sham exposed mice. In transgenic mice, no significant differences were found between the groups. Moreover, no significant differences were found between groups co-exposed to UV radiation and a magnetic field (groups 5 and 6) and exposure to UV radiation alone.
The authors conclude that an acute exposure of mice to a 50 Hz magnetic field could affect the epidermal level of polyamines and ornithine decarboxylase enzyme activity and thus augment tumorigenesis.

研究の種別:

研究助成

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