研究のタイプ: 医学/生物学の研究 (experimental study)

[電磁界はラットの骨髄間葉系幹細胞の骨芽細胞への分化を促進し、脂肪細胞への分化を阻害する] med./bio.

EMF acts on rat bone marrow mesenchymal stem cells to promote differentiation to osteoblasts and to inhibit differentiation to adipocytes.

掲載誌: Bioelectromagnetics 2010; 31 (4): 277-285

この研究は、低周波磁界(15 Hz, 1mT)が骨髄間葉幹細胞(MSCs)の骨芽細胞および脂肪細胞への分化を直接的に制御するか否かをラットのMSCsを用いて調べた。磁界ばく露の結果として、幹細胞骨芽細胞への分化促進され、同時に脂肪細胞形成が阻害された;アルカリホスファターゼ(ALP)活性と石灰化小節形成が増加し、RUNX2、ALP、BMP2、DLX5、BSPなど骨芽細胞固有mRNAの発現刺激された;反対に、脂質生成が減少し、アジプシン、AP-2、PPARγ2など脂肪細胞固有mRNAの発現が阻害され、またPPARγ2のタンパク質発現も阻害された、などの所見を報告している。

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研究目的(著者による)

To study the role of sinusoidal electromagnetic fields of 15 Hz (1 mT) on rat mesenchymal stem cell differentiation (adipogenesis and osteogenesis) and thus, to evaluate the possible mechanism of electromagnetic fields in prevention or treatment of osteoporosis.

詳細情報

The bone marrow contains mesenchymal stem cells that exhibit the ability to differentiate into adipocytes and osteoblasts. A reciprocal relationship exists between bone marrow mesenchymal stem cells adipogenesis and osteogenesis. The balance between this osteoblast and adipocyte differentiation is disrupted in various human diseases.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 15 Hz
ばく露時間: continuous for 8 h/day on 3 days
Alkaline Phosphatase Activity
ばく露2: 15 Hz
ばく露時間: continuous for 15 days or 21 days
Mineralized Bone Matrix Formation
ばく露3: 15 Hz
ばく露時間: continuous for 21 days
Assessment of Cytoplasmic Lipid Droplet Formation
ばく露4: 15 Hz
ばく露時間: continuous for 14 days
Immunoblot Analysis

ばく露1

主たる特性
周波数 15 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 8 h/day on 3 days
Additional information Alkaline Phosphatase Activity
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 pair of Helmholtz coils with a diameter of 30 cm, positioned 30 cm apart, placed inside an incubator; culture dishes positioned between the coils; EMF uniformity about 90 % in the exposure area
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - - - -

ばく露2

主たる特性
周波数 15 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 15 days or 21 days
Additional information Mineralized Bone Matrix Formation
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - - - -

ばく露3

主たる特性
周波数 15 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 21 days
Additional information Assessment of Cytoplasmic Lipid Droplet Formation
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - - - -

ばく露4

主たる特性
周波数 15 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 14 days
Additional information Immunoblot Analysis
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The data showed that the electromagnetic field promoted osteogenic cell differentiation of the stem cells and concurrently inhibited adipocyte formation. The electromagnetic field increased alkaline phosphatase enzyme activity and mineralized nodule formation, and stimulated osteoblast-specific gene expression of RUNX2, ALP, BMP2, DLX5, and BSP. In contrast, the electromagnetic field exposure decreased adipogenesis and inhibited adipocyte-specific gene expression of adipsin, AP2, and PPARγ2, and also inhibited protein expression of PPARγ2.
These findings suggest that commitment of mesenchymal stem cells into osteogenic or adipogenic lineages is influenced by the electromagnetic field.

研究の種別:

研究助成

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