EMF acts on rat bone marrow mesenchymal stem cells to promote differentiation to osteoblasts and to inhibit differentiation to adipocytes med./bio.

[EMF wirkt auf mesenchymale Knochenmarks-Stammzellen der Ratte, indem es die Differenzierung in Osteoblasten fördert und die Differenzierung die Adipozyten unterdrückt]

Ziel der Studie (lt. Autor)

Hintergrund/weitere Details

Endpunkt

• Zellfunktionen: Zelldifferenzierung (Adipogenese, d.h. Bildung von Adipozyten (Fettzellen) und Osteogenese)

Exposition/Befeldung (teilweise nur auf Englisch)

• • magnetisches Feld
  • Niederfrequenz

Vollständige Ansicht Kompaktansicht

Methoden Endpunkt/Messparameter/Methodik

• molekulare Biosynthese: Genexpression von Adipozyten-spezifischen Genen (Adipsin, AP2, PPARγ2) und von Osteogenese-spezifischen Genen (ALP, BMP2, DLX5, RNUX2 und BSP) (Real-time RT-PCR); Proteinexpression von PPARγ2 (Western Blot)
• Zellfunktionen: Zelldifferenzierung (Adipogenese und Osteogenese); alkalische Phosphatase-Enzymaktivität (Marker für die osteogene Differenzierung; Absorptions-Messung)
• morphologische/histopathologische Veränderungen: Osteogenese: extrazelluläre Matrix-Mineralisierung (als Marker für die Knochen-Zell-Reifung; von Kossa-Färbung von Mineral (Calcium-Phosphat)-Ablagerungen); Adipogenese: zytoplasmatische Lipid-Tröpfchen-Bildung (Oil Red O-Färbung)

Hauptergebnis der Studie (lt. Autor)

Studie gefördert durch

• National Natural Science Foundation (NSFC), China

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