研究のタイプ: 医学/生物学の研究 (experimental study)

[磁界による出芽酵母増殖の変化] med./bio.

Changes in Saccharomyces cerevisiae development induced by magnetic fields.

掲載誌: Biotechnol Prog 2001; 17 (5): 970-973

この研究は、110 mTおよび220 mTの定常磁界SMFばく露を受けた出芽酵母(Saccharomyces cerevisia)で最終的に誘発される増殖および代謝活性の変化を観察した。観察の開始時と終了時に、細胞質量(バイオマス)の増殖を分光法で測定し、代謝の変化を、産生CO2圧および培地pHの変化から推定した。非通気の液体寒天サブローブドウ糖培地培養中の酵母株DAUFPE-1012に、NdFeBr磁石によって生成されたSMFのばく露を行った。その結果、220 mTのSMFばく露群で、対照群と比較して、細胞増殖の増加(1.84%)およびCO2産生の増加(36.1%)が観察された;220 mTの SMFばく露群における培地の、実験開始から終了までの間におけるpHの差は、110 mTのSMFばく露群および対照群より大きかった;110 mT群および対照群に比べると、220 mT群でばく露後に観察された酸性化およびCO2産生の増加はバイオマス増加値に対応しないことから、これは細胞代謝速度の増強によって引き起こされたように見える、と報告している。

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研究目的(著者による)

To examine growth alterations and changes in metabolic activity in the yeast Saccharomyces cerevisiae exposed to a static magnetic field.

詳細情報

The cell number of Saccharomyces cerevisiae can be determined by measuring the absorbance, the so-called optical density. The metabolic activity can be estimated on the basis of the CO2 release and the pH value of the media. A high CO2 release and a low pH value indicate a high metabolic activity.
Eight flasks were filled with 10 ml culture medium and inoculated with a cell suspension. Every two hours in four flasks the biomass growth and in the other four flasks the CO2 pressure was measured. The pH value was determined at the beginning and at the end of the experiment. The cell cultures were exposed or sham exposed at 110 mT or 220 mT.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1:
  • DC/static
ばく露時間: continuous for 24 h

ばく露1

主たる特性
周波数
  • DC/static
タイプ
  • magnetic field
ばく露時間 continuous for 24 h
ばく露装置
ばく露の発生源/構造
  • permanent NdFeB magnets
ばく露装置の詳細 round permanent NdFeB magnets were inserted into an Isopor polyurethane foam plate; to achieve the 220 mT static magnetic field two magnets were inserted into the plate at each side of the flasks; the 110 mT static magnetic field was obtained by placing one magnet into a fitting round shaped slit just under the center of the flasks
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 220 mT peak 測定値 - inside the flasks
磁束密度 110 mT minimum 測定値 - inside the flasks

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露前
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

In the 220 mT exposed group, a slightly increased biomass growth was observed and the CO2 pressure was increased in comparison to the control group and the 110 mT exposed group after 12 to 24 hours. The pH value at the end of the experiment was lower in the 220 mT exposed group when compared to the control group and the 110 mT exposed group.
The authors conclude that the observed alterations in the 220 mT exposed group were provoked by an enhancement in the cellular metabolic rate.

研究の種別:

研究助成

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