研究のタイプ: 医学／生物学の研究 (experimental study)

[ラジオ周波数照射はヒトTリンパ球とラット初期アストロサイトにストレス反応を誘導しない] med./bio.

Radiofrequency radiation does not induce stress response in human T-lymphocytes and rat primary astrocytes

著者: Lee JS, Huang TQ, Kim TH, Kim JY, Kim HJ, Pack JK, Seo JS
掲載誌: Bioelectromagnetics 2006; 27 (7): 578-588
RISフォーマットで参考文献をダウンロード

この研究は、細胞実験で、高周波（RF）電磁界が非熱的にストレス応答を誘導するか否かを調べた。ヒトTリンパ球ジャーカット細胞およびラット初代星状細胞に、1763 MHzのRF電磁界ばく露を、SARレベルは2 W / kgまたは20 W / kg、ばく露時間は30分または1時間で与えた。温度は、ばく露時間中37±0.2 ℃に制御された。ばく露終了後に、熱ショックタンパク質HSPの発現およびマイトジェン活性化プロテインキナーゼ（MAPKs）の活性化の変化を評価した。その結果、ばく露細胞において、HSP90、HSP70、およびHSP27の発現レベルに検出可能な差異は観察されなかった；MAPKs、細胞外シグナル調節キナーゼ（ERK1 / 2）、c-Jun N末端タンパク質キナーゼ（JNK1 / 2）、またはp38のリン酸化状態は有意に変化しなかった、と報告している。続いて、ストレス応答に対する12-O-テトラデカノイルホルボール13-アセテート（TPA）の効果をRF放射が促進できるかどうかを調べるために、TPA処理と組み合わせたRF放射ばく露実験を行なった。その結果、TPA単独処理の場合、MAPKは用量依存的にリン酸化された；しかし、RF放射はTPA誘発MAPKリン酸化の増強をもたらさなかった；TPAもRF放射も、HSPの誘導に検出可能な影響を及ぼさなかった、と報告している。

The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →

研究目的（著者による）

To study the effects of 1763 MHz irradiation emitted from mobile phones on stress response.

詳細情報

Expression of heat shock proteins and the phosphorylation of the mitogen-activated protein kinases (MAPK; major participants in stress responses) were monitored. Whether radiofrequency exposure exerted a synergistic effect on the status of mitogen-activated protein kinase phosphorylation induced by TPA was also determined. Positive controls were performed (43°C, 30 min).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,762.5 MHz
ばく露時間: continuous for 30 min or 1 h
  • SAR: 2 W/kg mean
  • SAR: 20 W/kg mean

ばく露1

主たる特性
周波数 1,762.5 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 30 min or 1 h
Modulation
Modulation type cf. additional information
Additional information

CDMA signal
ばく露装置
ばく露の発生源／構造
チャンバの詳細 The experimental chamber (W 115 mm x H 80 mm x L 240 mm) was made of aluminium and therefore shielded. The temperature was maintained at 37 ± 0.2°C by cooling water circulating through its bottom. Gas from an incubator was circulated through the cavity to maintain CO2 density and humidity.
ばく露装置の詳細 The RF signal was fed by a λ/8 monopole antenna (22 mm long) at the λ/4 position of the top plate of the cavity exciting only the fundamental TE102 mode and allowing a uniform exposure of a 100-mm culture dish. The 100-mm Petri dish having an inner diameter of 85 mm, a 1-mm wall, and a 91-mm lid was filled with 18 ml of culture medium standing 4 mm high.
Sham exposure A sham exposure was conducted.
Additional information For positive (heat shock) control, culture dishes were wrapped tightly with parafilm and immersed in a water bath at 43 ± 0.2°C for 30 min.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg mean 測定値および計算値 - -
SAR 20 W/kg mean 測定値および計算値 - -
Additional parameter details

Cells were exposed to RF radiation either alone or in combination with TPA.

測定および計算の詳細

SAR was determined numerically using the FDTD technique and by measurements using fluoroptic temperature probes with a thermal resolution of 0.1 °C. The temperature rise was measured at nine positions inside the Petri dish. The results of both methods were in excellent agreement.

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

No detectable difference was found in the expression levels of the different heat shock proteins of exposed cells as compared to the levels measured in sham-exposed cells. The phosphorylation status of mitogen-activated protein kinases, extracellular signal-regulated kinases, c-Jun N-terminal protein kinases, or p38, did not change significantly.
When TPA alone was applied, the mitogen-activated protein kinases were found to be phosphorylated in a dose-dependent manner. However, radiofrequency irradiation did not result in any enhancement of TPA-induced phosphorylation. Neither TPA nor radiofrequency irradiation exerted any detectable effect on the induction of heat shock proteins.

研究の種別:

研究助成

関連論文