研究のタイプ: 医学/生物学の研究 (experimental study)

[無線周波(900 MHz)の放射はヒト神経芽腫細胞におけるEgr-2遺伝子発現を誘発し、細胞周期制御に影響をもたらす] med./bio.

Radiofrequency radiation (900 MHz) induces Egr-1 gene expression and affects cell-cycle control in human neuroblastoma cells

掲載誌: J Cell Physiol 2007; 213 (3): 759-767

イオン放射線、紫外線を含む多くの環境シグナルはEgr-1遺伝子の活性化を誘発し、それによって細胞増殖アポトーシスに影響を及ぼす。神経細胞電磁界(EMF)ばく露の影響に関する知識は不十分で議論の余地があることから、SH-SY5Y神経芽腫細胞における無線周波(RF)放射線の生物的影響を調査することになった。Egr-1遺伝子発現におけるワイヤーパッチセル(WPC)アンテナばく露システムによる900MHzの変調無線周波界の影響は、時間関数として研究された。短期間のばく露は、MAPK(分裂促進因子活性化タンパク質キナーゼ)亜類型EPK1/2とSAPK/JNKの活性化と並行して、Egr-1 mRNAレベルでの一時的増加を誘発した。細胞増殖速度とアポトーシスにおける無線周波放射線の影響も研究された。無線周波放射線へのばく露は、24時間で観察された重要な影響では、SH-SY5Y細胞での増殖抑制作用を示した。無線周波放射線は顕著なG2-M阻害に達して、細胞周期進行を弱めた。さらに、Bcl-2と残存遺伝子のmRNAレベルでの大きな減少を伴い、またG2-M阻止およびアポトーシスとの信号伝達は妨害されて、アポトーシスの特徴であるsub-G1ピークの出現が24時間ばく露後に顕著になった。我々の結果は、900MHz-変調無線周波放射線へのばく露は、Bcl-2や残存遺伝子のようなアポトーシス阻害物に関連するEgr-1遺伝子発現および細胞調節機能に影響を与える証拠を提示し、インビトロ細胞のバイアビリティを制御する広範囲なメカニズムへの影響可能性の重要な洞察を提示した。

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研究目的(acc. to editor)

This study was conducted to determine the effects of a 900 MHz-modulated radio frequency field on viability in human neuroblastoma cells in vitro.


The cell viability was assessed by cell proliferation, cell cycle progression, expression studies of genes involved in cell proliferation and apoptosis (egr-1, p53, apoptosis inhibitor: bcl-2, survivin) up to chrarcterization of molecular processes via enzyme activity. The investigated transcription factor egr-1 is a gene essential for cell proliferation, cell differentiation, and apoptosis. Increased egr-1 expression and the induced Egr-1 protein is a strong transcriptional activator of key genes involved in cell death pathway.



ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 5, 15, 30 min, 6 or 24 h

General information

Lovisolo GA, Asta D, Ciammetti L, Mancini S, Marino C, Pinto R, D'Inzeo G. 2000. In vitro exposure system operating at 900 and 1800 MHz. In: Kostarakis P, Stavroulakis P, editors. Proceeding of Millennium International Workshop on Biological Effects of Electromagnetic field. Heraklion, Crete, Greece, 17-20 October, pp 169-175.


周波数 900 MHz
  • electromagnetic field
ばく露時間 continuous for 5, 15, 30 min, 6 or 24 h
Modulation type pulsed
Duty cycle 12.5 %
Additional information

GSM modulated

  • WPC antenna and signal generator with Proportional Integral Derivative algorithm to stabilize power
チャンバの詳細 The RF exposure system was based on a wire patch cell (WPC) antenna [Laval et al., 2000] and assembled as described in [Lovisolo et al., 2000]. Two WPCs were located in the same incubator for blinded RF and sham exposure. The absence of any interference between the two WPCs was experimentally confirmed.
Sham exposure A sham exposure was conducted.
Additional information Sixteen hours prior to RF exposure, cells in logarithmic growth were aliquoted from a single parental flask into individual 35-mm Petri dishes. The temperature of the dishes was maintained at 37 ± 1 °C by an appropriate cooling system [Ardoino et al., 2004]. The exposure system was turned on at least 30 min before the experiment, and the temperature in the incubator was monitored with a thermocouple probe (sensitivity ± 0.1 °C) during the exposure.
測定量 種別 Method Mass 備考
電界強度 23 V/m effective value 計算値 - -
SAR 1 W/kg mean 計算値 - -

Reference articles

  • Merola P et al. (2006): [900 MHz変調ラジオ周波数電磁界にばく露された神経芽様細胞株の増殖とアポトーシス]
  • Ardoino L et al. (2004): [モバイル通信システムの影響に関する実験研究のための1800 MHzインビトロばく露装置]
  • Laval L et al. (2000): [900 MHzの携帯電話周波数用の新しいIn vitro用ばく露装置]


方法 影響評価項目/測定パラメータ/方法

  • ばく露前
  • ばく露中
  • ばく露後


The exposure to radiofrequency field for 24 hours exerted a significant reduction in cell viability and cell proliferation. Within exposure the egr-1- gene expression increased in 5 minutes, reaching maximum after 15 minutes, and decline to baseline levels after 6 hours. The enzyme activities of the MAPK subtypes showed similar pattern.
Cells exposed for 24 hours exhibited cell cycle progress typical for apoptosis accompanied by significant decreased gene expression of the investigated apoptosis inhibitor bcl-2 and survivin.