研究のタイプ: 医学/生物学の研究 (experimental study)

[イン・ビトロでのヒト線維芽細胞および形質転換GFSH-R17ラット顆粒膜細胞における携帯電話放射(1800MHz)による非熱的なDNA切断] med./bio.

Non-thermal DNA breakage by mobile-phone radiation (1800 MHz) in human fibroblasts and in transformed GFSH-R17 rat granulosa cells in vitro.

掲載誌: Mutat Res Genet Toxicol Environ Mutagen 2005; 583 (2): 178-183

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研究目的(著者による)

To confirm or disprove the genotoxic potential of radiofrequency electromagnetic field by investigating possible DNA-damaging effects in different cell types by using a newly developed sophisticated exposure system (publication 7926).
Information of the Medical University of Vienna (23.05.2008): Suspicion of an erroneous study by the former Division of Occupational Medicine (in German) and the press release Science and Ethics (in German) .
The Austrian Agency for Research Integrity was not able to verify the suspicion of an erroneous study (see statement of 23.11.2010).

詳細情報

The work is part of the REFLEX project (Risk Evaluation of Potential Environmental Hazards From Low Energy Electromagnetic Field Exposure Using Sensitive in vitro Methods), funded by the European Union.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: CW
ばく露時間: continuous for 4, 16 and 24 h
  • SAR: 2 W/kg mean
ばく露2: 1,800 MHz
Modulation type: CW
ばく露時間: intermittent, 5 min on/10 min off, for 4, 16 and 24 h
  • SAR: 2 W/kg mean
ばく露3: 1,800 MHz
Modulation type: pulsed
ばく露時間: intermittent, 5 min on/10 min off, for 4, 16 and 24 h
  • SAR: 2 W/kg mean
ばく露4: 1,800 MHz
Modulation type: pulsed
ばく露時間: continuous for 4, 16 and 24 h
  • SAR: 1.2 W/kg mean

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 4, 16 and 24 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • R18 hollow rectangular waveguides
チャンバの詳細 Two waveguides were placed inside a commercial incubator at 37°C, 5% CO2, and 95% humidity. A computer randomly determined which of the two waveguides was used for exposure (blind design).
ばく露装置の詳細 Six Petri dishes were exposed simultaneously in the waveguide and placed in the H-field maxima.
Additional information Each exposure level was tested in duplicate.
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg mean 測定値および計算値 - -

ばく露2

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 intermittent, 5 min on/10 min off, for 4, 16 and 24 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg mean 測定値および計算値 - -

ばく露3

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 intermittent, 5 min on/10 min off, for 4, 16 and 24 h
Modulation
Modulation type pulsed
Pulse width 576 µs
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
Additional information

GSM basic signal having a frame length of 4.61 ms. Since every 26th frame was idle, an 8 Hz modulation component was integrated into the signal.

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
SAR 2 W/kg mean 測定値および計算値 - -

ばく露4

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 4, 16 and 24 h
Modulation
Modulation type pulsed
Pulse width 576 µs
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
Additional information

GSM talk signal alternating between GSM basic (97 s) and DTX mode (50 s) simulating a conversation.

ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
SAR 1.2 W/kg mean 測定値および計算値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Electromagnetic field exposure (SAR 1.2 or 2 W/kg; different modulations; during 4, 16 and 24 h; intermittent or continuous wave) induced DNA single-strand breaks and double-strand breaks. The evaluation became significant after 16 h of exposure. Effects occurred (after 16 h irradiation) in both cell types and after different modulations. The intermittent exposure showed a stronger effect than continuous exposure.
The authors conclude that the induced DNA damage cannot be based on thermal effects.

研究の種別:

研究助成

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