研究のタイプ: 医学/生物学の研究 (experimental study)

[2.45GHzの無線周波電磁界は培養ヒト細胞における遺伝子発現を変化させる] med./bio.

2.45 GHz radiofrequency fields alter gene expression in cultured human cells.

掲載誌: FEBS Lett 2005; 579 (21): 4829-4836

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研究目的(著者による)

To study whether radiofrequency fields can cause changes in gene expression in cultured human cells. The authors aimed to provide genome-wide coverage of the expressed genes regardless their functional categories in the radiofrequency treated cells to address if radiofrequency has biological effects.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2.45 GHz
Modulation type: pulsed
ばく露時間: continuous for 2 or 6 h

General information

The RF exposure system has been described in detail [Gerber et al., manuscript in preparation].

ばく露1

主たる特性
周波数 2.45 GHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 2 or 6 h
Modulation
Modulation type pulsed
Pulse width 155 µs
Duty cycle 7.5 %
ばく露装置
ばく露の発生源/構造
  • 導波管
  • 86.36 x 43.18 mm, pulsed magnetron
チャンバの詳細 The waveguide was filled with a 5% air-CO2 mixture through a hole in its top surface. The bottom of the waveguide was maintained at 37 °C by temperature-controlled water circulating in direct contact with the brass surface through an exterior plastic channel glued to it.
ばく露装置の詳細 A 25-ml culture flask containing 10 ml of cell suspension was placed inside the waveguide. The bottom of the flask was ground flat and coated with mineral oil to obtain good thermal conduction. The temperature in the bottom surface of the flask monitored by two Luxtron probes was maintained at 37.2 ± 0.2 °C.
Sham exposure A sham exposure was conducted.
Additional information The cells were allowed to settle down to the bottom of the flask to form a monolayer before exposure. Sham exposure was performed in an identical waveguide for 2 h only.
パラメータ
測定量 種別 Method Mass 備考
SAR 10 W/kg mean 電力損失による決定値 - -
SAR 133 W/kg peak 計算値 - -
電界強度 320 V/m peak 計算値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The authors revealed that 221 genes altered their expression after a 2 h exposure. The number of affected genes increased to 759 after a 6 h exposure. Functional classification of the affected genes showed that apoptosis-related genes were among the upregulated ones and the cell cycle genes among the downregulated ones. No significant increase in the expression of heat shock protein genes was found.
These data suggest that the radiofrequency fields at 2.45 GHz can alter gene expression in cultured human cells through non-thermal mechanism.

研究の種別:

研究助成

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