研究のタイプ: 医学/生物学の研究 (experimental study)

[大腸菌及び黄色ブドウ球菌の生存力に対する50 Hzの回転磁界の影響] med./bio.

Effects of 50 Hz rotating magnetic field on the viability of Escherichia coli and Staphylococcus aureus.

掲載誌: Electromagn Biol Med 2014; 33 (1): 29-34

この研究は、3個のコイルを互いに120度ずつずらして配置し、50Hzの3相交流によりコイル内部に発生させた、30mTの50Hz回転磁界(RMF)へのばく露大腸菌および黄色ブドウ球菌の生存力に及ぼす影響を、さまざまなばく露時間(30~270分間)およびばく露終了後の経過(270分後まで)について調べた。バクテリア試料10mlは15mlのプラスティック製試験管に入れ、試験管をコイル内の30mT均一磁界区域に留置した。その結果、RMFばく露は、調べた2種類のバクテリア試料全てにおいて、増殖および細胞代謝活性の総体を示すCD(光学密度)値を増加させた(特にばく露時間30、60、90分では両バクテリアとも増加したが、150分では黄色ブドウ球菌のみ増加した);60分間ばく露の終了後の経過では、黄色ブドウ球菌は高いCD値が続いたが、大腸菌は90分後から急速に低下した、と報告している。

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研究目的(著者による)

To investigate the influence of a 50 Hz-rotating magnetic field on the cell proliferation and cell viability of the two bacteria species Escherichia coli and Staphylococcus aureus depending on the exposure duration.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for up to 270 minutes (30, 60, 90, 120, 150, 180, 210, 240 or 270 minutes)

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for up to 270 minutes (30, 60, 90, 120, 150, 180, 210, 240 or 270 minutes)
ばく露装置
ばく露の発生源/構造
チャンバの詳細 bacteria were exposed in 15 ml plastic tubes
ばく露装置の詳細 setup consisted of a generator made of a stator of a three-phase asynchronical engine and a glass container filled with water (served as water bath for the test tubes); stator was made of stampings with slots to carry three-phase winding that were geometrically spaced 120° apart; generator produced electromotive force in the bulk of the glass container; magnetic field was generated by coils located around the cylinder and rotates about the cylinder axis with a constant angular frequency; temperature of water bath was 37°C ± 0.5°C
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 30 mT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In exposed cell cultures, the cell proliferation and the cell viability was significantly increased compared to the control group. However, the observed effects were limited up to the time-point of 150 minutes.
The authors conclude that the observed stimulation of cell proliferation and cell viability in Escherichia coli and Staphylococcus aureus due to 50 Hz magnetic field exposure could have a negative influence in the case of pathogen treatment, but could have also a beneficial effect in biotechnologic cultivation methods.

研究の種別:

研究助成

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