研究のタイプ: 医学/生物学の研究 (experimental study)

[異なる種のバクテリアの機能的パラメータに対する回転磁界ばく露の影響] med./bio.

Effects of rotating magnetic field exposure on the functional parameters of different species of bacteria.

掲載誌: Electromagn Biol Med 2015; 34 (1): 48-55

この研究は、9種類の細菌増殖細胞代謝活動、バイオフィルム形成に対する回転磁界(RMF)の影響を調べた。用いた細菌は、黄色ブドウ球菌(S. aureus)、大腸菌(E. coli)、アネシストバクター属菌(A. baumannii)、緑膿菌(P. aeruginosa)、セラチア菌(S. marcescens)、ミュータンス菌(S. mutans)、クロノバクター属菌(C. sakazakii,)、肺炎病原菌(K. oxytoca)、表皮ブドウ球菌(S. xylosus)である。RMFのばく露条件は、B = 25-34 mT、周波数5-50 Hzばく露時間60分間としたほか、一定のばく露(34 mT、50 Hzばく露時間60分間)後に300分間培養して、影響の持続性も調べた。その結果、黄色ブドウ球菌大腸菌、セラチア菌、ミュータンス菌、クロノバクター属菌、肺炎病原菌、表皮ブドウ球菌では、RFRによる刺激が見られたが、アネシストバクター属菌、緑膿菌代謝活動およびバイオフィルム形成は抑制された、と報告している。

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研究目的(著者による)

The effects of exposure of different species of bacteria to several extremely low frequency magnetic fields on growth, metabolic activity and biofilm formation should be investigated.

詳細情報

A total of 9 different bacteria species (see "exposed system") with different gram staining characteristics and shapes were used. Bacteria of each species were assigned to the following groups: 1) exposure to a 5 Hz magnetic field with 25 mT, 2) exposure to 25 Hz magnetic field with 29 mT, 3) exposure to a 50 Hz magnetic field with 34 mT, 4) exposure to a 50 Hz magnetic field with 34 mT and further incubation without exposure for 300 minutes, 5) sham exposure and 6) control group. The control group was conducted simultaneously to an actual exposure in the reserve water bath, which was used to maintain the temperature in the exposure generator.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 5 Hz
ばく露2: 25 Hz
ばく露3: 50 Hz

ばく露1

主たる特性
周波数 5 Hz
タイプ
  • magnetic field
Additional information rotating magnetic field
ばく露装置
ばく露の発生源/構造
チャンバの詳細 15 ml polypropylene tubes
ばく露装置の詳細 setup contained generator for the field, which was made of a three-phase stator of an induction squirrel cage motor, and a water bath incubator (glass container) for the tubes with bacteria placed inside it during the exposure; the glass container was axially aligned with the generator and positioned symmetrically with respect to its lower and upper ends; temperature in the water bath remained constant at 37 ± 0.5°C
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 25 mT mean 測定値および計算値 - ± 0.5 mT

ばく露2

主たる特性
周波数 25 Hz
タイプ
  • magnetic field
Additional information rotating magnetic field
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 29 mT mean 測定値および計算値 - ± 0.5 mT

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
Additional information rotating magnetic field
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 34 mT mean 測定値および計算値 - ± 0.5 mT

Reference articles

  • Nawrotek P et al. (2014): [大腸菌及び黄色ブドウ球菌の生存力に対する50 Hzの回転磁界の影響]
  • Rakoczy R et al. (2011): [横方向の回転磁界により誘導される混合プロセスの研究]
  • Masiuk M et al. (2008): [回転磁界に反復的に短時間ばく露した後のHL-60およびK-562細胞でのヌクレオリンの発現および核内分布]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Growth rate, metabolic activity and biofilm formation were significantly increased in S. aureus, E. coli, S. marcescens, S. mutans, C. sakazakii, K. oxytoca and S. xylosus cultures compared to the control group immediately after exposure. However, the metabolic activity and biofilm formation were significantly impaired in A. baumannii and P. aeruginosa cultures. The most distinct effects were found after an exposure to the 50 Hz field with 34 mT (group 3).
300 minutes after exposure (group 4), no significant differences were detected in comparison to the control group anymore. Also no significant differences could be found between the sham exposure group and control group.
The authors conclude that exposure of bacteria to extremely low frequency magnetic fields could affect growth, metabolic activity and biofilm formation depending on the exposed species and frequency and strength of the field.

研究の種別:

研究助成

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