研究のタイプ: 医学／生物学の研究 (experimental study)

[メラトニンはラットの脳及び後根神経節におけるTRPM2及び電位依存性Ca2+チャネルを通じて無線（2.45 GHz）誘導性の酸化的損傷を調節する] med./bio.

Melatonin modulates wireless (2.45 GHz)-induced oxidative injury through TRPM2 and voltage gated Ca(2+) channels in brain and dorsal root ganglion in rat

著者: Naziroglu M, Celik O, Ozgul C, Cig B, Dogan S, Bal R, Gumral N, Rodriguez AB, Pariente JA
掲載誌: Physiol Behav 2012; 105 (3): 683-692
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この実験研究は、ラットの脳および後根神経節（DRG）のニューロンにおける抗酸化還元システム、Ca2+流入、細胞バイアビリティ、脳電図（EEG）記録に対する2.45GHz電磁界（EMR）ばく露の影響とそれに対するメラトニンの保護効果を調べた。32匹のWistarアルビノ雄ラットを無作為に4群（各8匹。A1：ケージ対照群、A2：擬似ばく露群、B：EMR群、C：EMR+メラトニン群）に分けた。EMR群は、60分/日で30 日間ばく露。ばく露装置は、モノポールアンテナの周囲に8匹のラットの頭部を中心に向けて放射状に固定して並べたもの（頭とアンテナの距離1cm、SARは約0.14W/kgと計算した）。C群は、B群と同じEMRばく露の他に、腹腔内注射によりメラトニン10 mg/kg/日を投与した。実験終了後、各群についてEEG測定した後、屠殺して脳とDRGの標本を採取した。その結果、DRGニューロンの脂質過酸化、細胞バイアビリティ、細胞内Ca2+レベルはA1、A2群よりB群の方が高かった；EEGのスパイク数はB群よりC群の方が少なかった；皮質のビタミンE濃度はB群よりC群の方が高かったなどの所見を報告している。

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研究目的（著者による）

To investigate the effects of a 2.45 GHz electromagnetic field exposure on the brain cortex and the dorsal root ganglion of rats, as well as the possible protective effects of melatonin administration.

詳細情報

4 groups (n=8 per group) were examined: 1.) cage control, 2.) sham exposure + daily injection of saline, 3.) exposure and 4.) exposure + daily injection of melatonin (10 mg/kg).
Before the animals were killed, an EEG was recorded. Afterwards, brain homogenates and the dorsal root ganglion were investigated for signs of oxidative stress. Additionally, in the dorsal root ganglion, the intracellular calcium concentration was determined and the patch-clamp technique was conducted.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2.45 GHz
Modulation type: pulsed
ばく露時間: continuous for 1 h/day on 30 days
  • 電界強度: 11 V/m
  • 電力密度: 1 mW/m²
  • SAR: 0.143 W/kg (at the rat's head with E = 10 V/m d: am Kopf der Ratte bei)
  • SAR: 0.1 W/kg spatial average (whole body)

General information

Rats were treated in the following four groups: i) cage control ii) sham exposure iii) EMF exposure iv) EMF exposure + melatonin administration (10 mg/kg per day)

ばく露1

主たる特性
周波数 2.45 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 1 h/day on 30 days
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源／構造
Distance between exposed object and exposure source 1 m
ばく露装置の詳細 rats kept in 15 cm long cylindrical constrainers with a diameter of 5 cm; 8 constrainers positioned radially on a groundplate with the antenna in the center; rats placed with their heads near the antenna
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
電界強度 11 V/m - 測定値 - -
電力密度 1 mW/m² - - - -
SAR 0.143 W/kg - 計算値 whole body at the rat's head with E = 10 V/m d: am Kopf der Ratte bei
SAR 0.1 W/kg spatial average 計算値 whole body -

Reference articles

  • Naziroglu M et al. (2009): [ラットの脳内における無線機器（2.45 GHz）由来の酸化ストレス及び脳電図記録にL-カルニチン及びセレンが及ぼす変調作用]
  • Gumral N et al. (2009): [無線装置からの2.45GHz放射によるラット血液中の酸化ストレスに対するセレンおよびL-カルニチンの効果]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

In general, no differences were found between the cage control (group 1) and the sham exposed group (group 2).
In the exposed group (group 3), the number of spikes in the EEG was increased compared to the control groups (group 1+2). However, no differences occurred in the EEG between the exposed group treated with melatonin (group 4) and the control groups (groups 1+2).
In the dorsal root ganglia of exposed rats (group 3), the cell viability was significantly decreased compared to the control groups (group 1+2). In contrast, there was no difference in the cell viability between the melatonin treated exposure group (group 4) and the control groups (groups 1+2). Additionally, in the exposed group (group 3), the level of lipid peroxidation and the intracellular calcium release were increased in comparison to the control groups (groups 1+2). Again, no differences in the level of lipid peroxidation and the intracellular calcium release were found between the melatonin treated exposure group (group 4) and the control groups (group 1+2). The current densities of the neurons were higher in the exposed group (group 3) than in the control groups (groups 1+2).
In brain homogenates of exposed rats, treated with melatonin (group 4), an increased concentration of vitamin E was found, whereas the concentration of vitamin E in the other groups (groups 1+2+3) did not change.
Remaining brain homogenate and dorsal root ganglion antioxidant values were not affected by exposure or melatonin administration.
The authors conclude that exposure to a wireless device of 2.45 GHz could induce oxidative stress in the dorsal root ganglion and that melatonin could prevent such a reaction.

研究の種別:

研究助成

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