研究のタイプ: 医学/生物学の研究 (experimental study)

[ヒト胎児強膜線維芽細胞に対する超低周波電磁界の影響] med./bio.

Effects of extremely low frequency electromagnetic fields on human fetal scleral fibroblasts.

掲載誌: Toxicol Ind Health 2016; 32 (6): 1042-1051

この研究は、ヒト胎児強膜線維芽細胞(HFSFs)に対する超低周波磁界(ELF-MF)の影響を調べた。ヘルムホルツコイルを用いて発生させたELF-MF(50Hz、磁束密度0.1、0.2、0.5、1.0mT)のばく露を、ばく露時間6-48時間で実施した。影響は、生存率および強膜構造に関与する因子で評価した。その結果、0.2 mTの24時間ばく露で、HFSFsの増殖速度が有意に低下した;コラーゲンタイプIのメッセンジャRNA発現減少およびマトリックスメタロプロテアーゼ2の発現増加が有意であった、などの所見を報告している。

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研究目的(著者による)

To investigate the effects of extremely low frequency magnetic fields on cell growth and factors involved in scleral structuring in scleral fibroblasts.

詳細情報

The possible impact of extremely low frequency magnetic fields on pathological alterations in the sclera has been rarely documented. However, it might play an important role in treating certain eye ailments (e.g. myopia) and therefore more research is required.
The sclera is a part of the human eye ("white of the eye"), mainly composed of collagen fibrils. Several factors can influence the correct formation and structure of these collagen fibrils. Therefore, the expression of several genes related to the collagen synthesis was examined.
The cells were exposed to a magnetic flux densitiy of 0.2 mT for 24 hours if not stated otherwise.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 24 hours
cell proliferation, protein expression, cell morphology
ばく露2: 50 Hz
ばく露時間: continuous for 24 hours
gene expression experiment 1
ばく露3: 50 Hz
ばく露時間: continuous for up to 48 hours (6, 12, 24, 36 or 48 hours)
gene expression experiment 2

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 24 hours
Additional information cell proliferation, protein expression, cell morphology
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 two exposure facilities (one for exposure, the other for sham exposure) with the same environment were put into two thermo-regulated and atmosphere-controlled incubators at 37°C and 5% CO2; the coils were 16 cm long, 14 cm wide and 8 cm high and consisted of 168 turns copper wire, respectively
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.2 mT - 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 24 hours
Additional information gene expression experiment 1
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.1 mT - 測定値 - -
磁束密度 0.2 mT - 測定値 - -
磁束密度 0.5 mT - 測定値 - -
磁束密度 1 mT - 測定値 - -

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for up to 48 hours (6, 12, 24, 36 or 48 hours)
Additional information gene expression experiment 2
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.2 mT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The cell proliferation of exposed cells was significantly decreased compared to the control group. Additionally, the morphology of exposed cells differed from those of the control group: While untreated cells appeared fibroblast-like (spindle or bundle shape), the alignment of exposed cells was altered into irregular polygonal configurations.
The gene expression of collagen 1 (starting after 6 hours) and fibroblast growth factor-2 (starting after 12 hours) was significantly decreased after exposure to 0.2 mT and higher magnetic flux densities in comparison to the control, while the gene expression of matrix-metalloproteinase-2 (starting after 24 hours; 0.1 and 0.2 mT) and transforming growth factor beta-2 (starting after 24 hours; 0.5 mT and 1 mT) was significantly increased. However, the gene expression level of the matrix-metalloproteinase-2 inhibitor was significantly increased only after exposure to 1 mT compared to the control.
The protein expression analysis was in agreement with the results of the gene expression examination: In exposed cells, the protein expression of collagen 1 was significantly decreased compared to the control while the protein expression of matrix-metalloproteinase-2 was significantly increased.
The authors conclude that extremely low frequency magnetic fields could influence the growth of scleral fibroblasts as well as its gene expression and protein expression related to the structure of the sclera.

研究の種別:

研究助成

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