研究のタイプ: 医学/生物学の研究 (experimental study)

[ラット腎臓でのMMP-2、MMP-9、IV型コラーゲンおよびE-カドヘリンの発現レベルに対するパルスおよび正弦波の電磁界の影響:免疫組織化学的研究] med./bio.

Effects of pulsed and sinusoidal electromagnetic fields on MMP-2, MMP-9, collagen type IV and E-cadherin expression levels in the rat kidney: an immunohistochemical study.

掲載誌: Anal Quant Cytopathol Histpathol 2013; 35 (5): 253-260

この研究は、ラットを用いた実験で、超低周波パルス電磁界および正弦波電磁界へのばく露腎臓組織に与える影響を調べた。27匹のオスのウィスターアルビノラットを3群(n = 9)に分けた。すなわち、対照群正弦波電磁界(SEMF;50 Hzばく露群、パルス電磁界PEMFパルス時間25 μs、50 Hzばく露群である。どちらの電磁界も、振幅は1.5 mT、ばく露時間は1日6時間、週5日、28日間であった。ばく露終了後、ホルマリン固定パラフィン包埋腎臓組織切片をヘマトキシリン-エオシン染色、ゴモリ法染色および過ヨウ素酸シッフ染色した。さらに、マトリックスメタロプロテイナーゼ-2(MMP-2)および9(MMP-9)、E-カドヘリンおよびコラーゲンタイプIVの発現レベルを免疫組織化学的に調べた。その結果:糸球体基底膜の肥厚が、両ばく露群、特にSEMF群で明白に見られた;さらに両ばく露群で、E-カドヘリン発現レベルは低下し、MMP-9の発現レベルは上昇したが、MMP-2およびIV型コラーゲン発現レベルは変化しなかった、と報告している。

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研究目的(著者による)

To investigate the effects of pulsed and sinusoidal magnetic fields on the protein expression levels of E-cadherin, type IV collagen, matrix metalloproteinase-2 and matrix metalloproteinase-9 as well as histopathological changes in the rat kidney.

詳細情報

Rats were divided into three groups (n=9 per group): 1.) cage control, 2.) exposure to a pulsed magnetic field and 3.) exposure to a sinusoidal magnetic field.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 6 h/day during 5 days a week for 28 days
pulsed
ばく露2: 50 Hz
ばく露時間: continuous for 6 h/day during 5 days a week for 28 days
sinusoidal

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • pulsed
ばく露時間 continuous for 6 h/day during 5 days a week for 28 days
Additional information pulsed
Additional information pulse time 25 µs
ばく露装置
ばく露の発生源/構造
チャンバの詳細 rats were exposed in methacrylate cages
ばく露装置の詳細 two pairs of Helmholtz coils in a Faraday cage (130 x 65 x 80 cm), magnet was constructed by winding 125 turns of insulated soft copper wire with adiameter of 1.5 mm, coils were placed vertically facing one another, distance between coils was 47 cm
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.5 mT - 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 6 h/day during 5 days a week for 28 days
Additional information sinusoidal
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1.5 mT - - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
  • kidneys
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In the cage control group, the histological examination revealed a normal structure of the kidneys. However, in the group exposed to pulsed magnetic fields, degenerative changes were observed in the renal tubuli as well as a thickening of the basement membranes and hemorrhage. In the group exposed to sinusoidal magnetic fields, hypertrophy of the glomeruli, narrowing of Bowman's space, inflammatory cell infilitration, degeneration of the renal tubuli and thickening of the basement membrane were found.
The protein expression levels of E-cadherin and matrix metalloproteinase-9 were significantly increased in both exposure groups in comparison to the cage control group. No significant differences were observed between the groups regarding the protein expression level of type IV collagen and matrix metalloproteinase-2.
The authors conclude that exposure to pulsed or sinusoidal magnetic fields could induce histopathological and molecular changes in the kidney of rats.

研究の種別:

研究助成

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