研究のタイプ: 医学/生物学の研究 (experimental study)

[磁界ばく露後の卵黄嚢の脈管構造におけるマルチフラクタル次元と空隙性] med./bio.

Multifractal dimension and lacunarity of yolk sac vasculature after exposure to magnetic field.

掲載誌: Microvasc Res 2015; 99: 1-7

この研究は、ウズラの胚の卵黄嚢(YSM)の脈管形成および血管新生に対する磁界ばく露MF:60Hz、1mT)の影響を、空隙性パラメータ(フラクタル解析の補完的方法)およびマルチフラクタル解析を用いた脈管構造の複雑性の評価により検討した。その結果、一日のばく露時間(6、9、12、14時間)により脈管構造の密度などに違いが見られたと報告している。

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研究目的(著者による)

It should be investigated if lacunarity and multifractal dimension analyses are suitable to measure the effects of exposure of embryos of the Japanese quail (Coturnix japonica) to a 60 Hz magnetic field on the development of the vascular system in the yolk sac membrane.

詳細情報

The study was based on Costa et al. 2013 and should refine the method of the monofractal analysis.
100 eggs of the Japanese quail were divided into 5 groups (n=20 each): 1) sham exposure, exposure to the magnetic field 2) for 3 x 2 h/day, 3) for 3 x 3 h/day, 4) for 3 x 4 h/day and 5) for 24 h/day. 48 hours after the eggs had been laid, a small part of the egg shell was removed and replaced by transparent foil for observation and exposure started afterwards.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: continuous for 2 h, 3 times a day with intervals of 6 hours in between for 2 days
ばく露2: 60 Hz
ばく露時間: continuous for 3 h, 3 times a day with intervals of 5 hours in between for 2 days
ばく露3: 60 Hz
ばく露時間: continuous for 4 h, 3 times a day with intervals of 4 hours in between for 2 days
ばく露4: 60 Hz
ばく露時間: continuous for 24 h for 2 days

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 2 h, 3 times a day with intervals of 6 hours in between for 2 days
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 Helmholtz coils were placed in an incubator; probes of the teslameter were placed on the horizontal axis of each coil; eggs were placed along the horizontal axis between the two coils
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - 測定値 - -

ばく露2

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 3 h, 3 times a day with intervals of 5 hours in between for 2 days
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - 測定値 - -

ばく露3

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 4 h, 3 times a day with intervals of 4 hours in between for 2 days
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - 測定値 - -

ばく露4

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 24 h for 2 days
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - 測定値 - -

Reference articles

  • Costa EV et al. (2013): [超低周波磁界にばく露したニホンウズラの胚における胚外血管新生のフラクタル分析]

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

After 1 day of exposure, lacunarity of the vascular system was significantly higher in group 3 (3 x 3 h/day of exposure) compared to the sham exposure group. The multifractal analysis showed significant differences in the generalized dimensions spectrum in groups 2 (3 x 2 hours/day of exposure) and 3 compared to the sham exposure group after 1 day of exposure. Both results indicate differences in the development of the vascular system in the respective exposure groups compared to the sham exposure group.
Moreover, in all groups a dominance of the higher generalized dimensions could be detected after 1 and 2 days of exposure in the multifractal analysis, indicating a multifractal dimension of the vascular system and a relative dominance of small vessels in the yolk sac membrane.
The authors conclude that exposure of embryos of the Japanese quail (Coturnix japonica) to a 60 Hz magnetic field could inhibit the development of the vascular system in the yolk sac membrane and that lacunarity and multifractal dimensions analyses are appropriate and complementary methods for the measurement of this endpoint.

研究の種別:

研究助成

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