この研究は、著者の前報（ヒト神経芽細胞腫細胞株SH-SY5Yにおいて、超低周波磁界（ELF-MF）へのばく露8および15日目に小核出現頻度が上昇し、ゲノム不安定が誘導された可能性が示唆されたと報告）を踏まえ、フォローアップを45日目まで延長した実験を行った。SH-SY5Yは、細胞外スーパーオキシド産生剤メナジオン（MQ）の共ばく露の有り、無しのそれぞれの条件下で、50Hz、100μTのMFに24時間ばく露した。小核、活性酸素種（ROS）、脂質過酸化（LPO）を15、30、45日目に測定した。その結果、前報と一貫して、15日目に小核出現頻度が上昇した；30日目でも見られたが45日目には見られなかった；LPOレベルは30、45日目に低下した；前報と同じく、MQ共ばく露はMFの影響に関係しなかった；MFの遅延性の影響におけるROSの役割を調べるため、抗酸化剤アセチルシステイン（NAC）を磁界ばく露前に投与すると、NACは効果的に細胞ROSレベルを低下させ、ROS に対するMQの作用を抑制したが、MFの作用は阻止されなかった（MFの遅延性の影響にROS上昇は必要ないことが示唆された）、と報告している。
The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →
The effects of exposure of human neuroblastoma cells to a 50 Hz magnetic field on oxidative stress and DNA damage should be investigated.
The study based on the results of a previous study by the authors (Luukkonen et al. 2014) and should enlarge the data. Menadione is a free radical-producing DNA damaging agent, and the antioxidant N-acetylcysteine were used in the tests to assess the influence of reactive oxygen species.
Cells were divided into the following groups: 1) exposure to the magnetic field, 2) co-exposure to the magnetic field and menadione, 3) co-exposure to the magnetic field and N-acetylcysteine, 4) co-exposure to the magnetic field, menadione and N-acetylcysteine. For each group, a respective control group with the same treatment but without exposure to the magnetic field was used. Cells were investigated 15, 30 and 45 days after exposure.
3 experiments with 2-3 samples per group, respectively, were performed. Positive controls were used.
| ばく露 | パラメータ |
|---|---|
|
ばく露1:
50 Hz
ばく露時間:
continuous for 24 hours
|
|
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 24 hours |
| ばく露の発生源／構造 | |
|---|---|
| チャンバの詳細 | cell culture dishes in temperature-controlled cell culture incubator with 5% CO2 |
| ばく露装置の詳細 | a horizontal MF was generated by a pair of Helmholtz coils (340 mm Œ 460 mm, 220 mm distance between the coils) inside an incubator; all cell culture dishes were placed at the center of the coil system, where the magnetic field was homogenous |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 100 µT | - | 測定値 | - | - |
The DNA damage was significantly elevated in groups exposed to the magnetic field (groups 1-4) compared to the respective control groups after 15 and 30 days, but not after 45 days.
Lipid peroxidation was significantly decreased in MF-exposed groups 1-4 compared to the respective control groups after 30 and 45 days.
The amount of reactive oxygen species was significantly increased in groups 3 and 4, which were exposed to the magnetic field and treated with N-acetylcysteine or menadione and N-acetylcysteine, compared to their respective control groups.
The authors conclude that exposure of human neuroblastoma cells to a 50 Hz magnetic field could induce DNA damage. Consistently with the previous study, this effect did not depend on co-exposure to menadione and was not blocked by simultaneous exposure to the antioxidant N-acetylcysteine, indicating that the increase in reactive oxygen species is no causal mechanism of action.
このウェブサイトはクッキー（Cookies）を使って、最善のブラウジングエクスペリエンスを提供しています。あなたがこのウェブサイトを継続して使用することで、私たちがクッキーを使用することを許可することになります。
