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The cytotoxic and genotoxic effects of an exposure of human neuroblastoma cells to a 50 Hz magnetic field with and without co-exposure to hydrogen peroxide should be investigated.
Cells were exposed to the magnetic field or sham exposed for 48 h, then treated with different concentrations of hydrogen peroxide for 1 h and afterwards further exposed to the magnetic field or sham exposed for up to 72 h. The MTT assay was performed in 6 replicates, the gamma-H2AX detection was performed in 3 replicates.
ばく露時間: continuous for up to 120 h
|ばく露時間||continuous for up to 120 h|
|Additional information||a bipolar pulsed-square wave 50 Hz magnetic field was chosen with a duty cycle of 50%; rise time of the square, from peak to peak, was about 0.6 ms, resulting in an average rate of change of magnetic flux density of 3.3 T/s|
|チャンバの詳細||cells were exposed in 3cm petri dishes|
|ばく露装置の詳細||two sets of horizontal Helmholtz coils, each of 25 cm diameter, with 40 (20 + 20) turns that were double-wrapped in order to obtain wound (active coil) or counter-wound configuration (sham exposure); active and sham coils were maintained in the same 5% CO2 incubator with a constant temperature of 37°C; samples were placed within a virtual cylinder (about 11 cm in height, and 4 cm in diameter), centered with respect to the coil system|
|Sham exposure||A sham exposure was conducted.|
|磁束密度||1 mT||peak||測定値||-||± 2%|
No significant differences were found between exposed and sham exposed cells.
The authors conclude that there is no indication for cytotoxic and genotoxic effects of an exposure of human neuroblastoma cells to a 50 Hz magnetic field. A co-exposure to hydrogen peroxide had no influence on the effects of the magnetic field, either.