研究のタイプ: 医学/生物学の研究 (experimental study)

[初代培養海馬ニューロンでの超低周波電磁界誘導性神経毒性に対するハスの莢抽出成分プロシアニジンの神経保護効果] med./bio.

Neuroprotective effects of lotus seedpod procyanidins on extremely low frequency electromagnetic field-induced neurotoxicity in primary cultured hippocampal neurons.

掲載誌: Biomed Pharmacother 2016; 82: 628-639

この研究は、ラット海馬ニューロン初代培養株を用いた実験で、超低周波電磁界(ELF-EMFばく露ばく露条件は抄録に不記載)による影響に対するハスの莢抽出成分プロシアニジン(LSPCs)の保護効果とそのメカニズムを検討した。その結果、ELF-EMFばく露下で培養した細胞株では、対照に比べ、細胞生存率低下、アポトーシス細胞の増加が見られたが、LSPCs投与により、そのような影響が顕著に阻止されたことなどを報告している。

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研究目的(著者による)

The neurotoxic effects of exposure of primary cultured hippocampal neurons to a 50 Hz magnetic field and the protective effects of lotus seedpod procyanidins should be investigated.

詳細情報

Lotus seedpod procyanidins (LSPCs) possess a broad spectrum of biological, pharmacological and chemoprotective properties against free radicals and oxidative stress. In a previous study by the authors (Duan et al. 2013), it was found that LSPCs had a protective effect against oxidative damage after ELF-EMF exposure in in vivo experiments.
Cells were divided into 5 groups: 1) exposure to the magnetic field, 2) exposure to the magnetic field and treatment with 2.5 mg/ml LSPCs, 3) exposure to the magnetic field and treatment with 5 mg/ml LSPCs, 4) exposure to the magnetic field and treatment with 10 mg/ml LSPCs, 5) control group.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 90 minutes

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 90 minutes
ばく露装置
ばく露の発生源/構造
チャンバの詳細 cell culture plates
ばく露装置の詳細 a pair of Helmholtz coils (1.0 mm wire diameter, 9 cm internal diameter, 13 cm external diameter) in 400 turns, parallel to the ground, generated a homogeneous field
パラメータ
測定量 種別 Method Mass 備考
磁束密度 8 mT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Cell viability, SOD enzyme activity and mitochondrial membrane potential were significantly decreased in exposed cells (group 1) compared to the control group, while lipid peroxidation, calcium concentration and the content of reactive oxygen species were significantly increased. However, in exposed groups with LSPCs (group 2-4), all these parameters were significantly restored in a LSPC concentration-dependent manner compared with group 1.
In morphological investigations, cells of group 1 showed a rounder shape and fewer neurites as well as condensed and fragmented nuclei compared to the control group. In a combination with 10 mg/ml LSPCs (group 4), morphology was significantly improved.
DNA damage, apoptosis and necrosis were significantly increased in group 1 compared to the control group. In groups 3 (with 5 mg/ml LSPCs) and 4, these parameters were significantly reduced in a LSPC concentration-dependent manner compared to group 1.
Cell cycle analysis revealed a significantly decreased number of cells in G0 phase/G1 phase and a significant S phase arrest in group 1 when compared with the control group. The LSPCs groups 3-4 showed a concentration-dependent restoration of both parameters compared to group 1.
The protein expressions of Bcl-2 and Bcl-xl were significantly decreased and the levels of Bad and Bax significantly increased in group 1 compared with the control group. LSPCs treatment increased the levels of anti-apoptotic proteins Bcl-2 and Bcl-xl and decreased the levels of pro-apoptotic proteins Bad and Bax.
The authors conclude that exposure to a 50 Hz magnetic field might induce neurotoxicity in hippocampal neurons via oxidative stress and activation of mitochondrial apoptotic pathway and that lotus seedpod procyanidins might protect against these effects.

研究の種別:

研究助成

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