研究のタイプ: 医学／生物学の研究 (experimental study)

[50Hz磁界ばく露およびLPS刺激を受けたマウスマクロファージRAW264における細胞生存率、DNA一本鎖切断、および一酸化窒素産生の評価] med./bio.

Evaluation of cell viability, DNA single-strand breaks, and nitric oxide production in LPS-stimulated macrophage RAW264 exposed to a 50-Hz magnetic field

著者: Nakayama M, Nakamura A, Hondou T, Miyata H
掲載誌: Int J Radiat Biol 2016; 92 (10): 583-589
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この研究は、マウスマクロファージ様細胞株RAW264を用いて、細胞の酸化ストレスと磁界ばく露との相乗作用の可能性を検討した。50Hz磁界（0.5mT、24時間ばく露）および内毒素であるリポ多糖（LPS；10ng/ml、1時間刺激；一酸化窒素NOを産生させて酸化ストレスを与える）の両者の有無を組合せた実験を実施し、終了後に細胞生存率（トリパンブルー色素排除試験による）、DNA一本鎖切断（アルカリコメット試験による）、および酸化ストレス（NO産生）を評価した。その結果、LPSの同時刺激を受けたマクロファージではNO産生が大きく増加しており、そのような群においてのみ50Hz 磁界はDNA一本鎖切断を増加させ、細胞生存率を下げた；磁界の単独ばく露では、NO産生は変化しなかった、と報告している。

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研究目的（著者による）

The synergistic effects of exposure to a 50 Hz magnetic field and LPS-induced oxidative stress on DNA damage, cell viability and oxidative stress in macrophages should be investigated.

詳細情報

In a previous study by the authors (Nakayama et al. 2014), it was found that exposure to a 50 Hz magnetic field increased DNA damage in mouse fibroblasts. However, it had not been investigated whether this phenomenon had any relation to cellular oxidative stress. The authors anticipated that lipopolysaccharides (LPS)-stimulated macrophages might be a suitable model to investigate whether oxidative stress and magnetic fields have synergistic effects because these cells produce large amounts of nitric oxide, which is a source of oxidative stress.
Cells were divided into the following groups: 1) exposure to the magnetic field, 2) sham exposure, 3) exposure to the magnetic field and LPS treatment (10 ng/ml for 1 hour prior to MF exposure), 4) sham exposure and LPS treatment.
Positive control for DNA damage was conducted.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 24 hours

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 24 hours
ばく露装置
ばく露の発生源／構造
チャンバの詳細 petri dishes in coils inside CO2 incubators
ばく露装置の詳細 four square coils made up one Merritt coil, which was constructed with two electric wires wound in parallel around an aluminum frame; cells were exposed to vertically oriented field
Sham exposure A sham exposure was conducted.
Additional information one Merritt coil used for the exposure was placed in one incubator and another identical Merritt coil was used for sham exposure in another identical incubator; in the exposure coils, electric currents in the two wires ran in the same direction, in sham exposure, currents ran in the opposite direction; the difference in temperature in both Merritt coils was < 0.3°C during the period of exposure
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 500 µT - 測定値 - -
Additional parameter details

magnetic field in the sham exposure coil was 0.52 ± 0.01 µT

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Exposure to the magnetic field alone (group 1) had no significant effect on any parameter in comparison to the sham exposed group (group 2).
Treatment with LPS alone (group 4) led to a significantly decreased cell viability compared to sham exposed cells (group 2) and to increased amounts of DNA damage (but not significant) and nitric oxide (significance not stated).
Co-exposure to the magnetic field and LPS (group 3) resulted in significantly more DNA damage and a significantly decreased cell viability compared to a treatment with LPS alone (group 4), but nitric oxide levels were not changed.
The authors conclude that exposure to a 50 Hz magnetic field might increase DNA damage and decrease cell viability in LPS-treated macrophages, while nitric oxide levels are not influenced.

研究の種別:

研究助成

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