研究のタイプ: 医学/生物学の研究 (experimental study)

Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity. med./bio.

掲載誌: Biodiscovery 2012; 2 (2): e8925

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研究目的(著者による)

The effect of exposure of primary lymphocytes to a 50 Hz magnetic field on cell viability, DNA damage and apoptosis should be investigated.

詳細情報

Lymphocytes were isolated from sheep, cultivated and divided into the following groups: 1) exposure to a 5 µT magnetic field, 2) exposure to a 50 µT magnetic field, 3) exposure to a 100 µT magnetic field and 4) control group. Four independent experimental trials were conducted within each group.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: for 16 hours
ばく露2: 50 Hz
ばく露時間: for 16 hours
ばく露3: 50 Hz
ばく露時間: for 16 hours

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 for 16 hours
ばく露装置
ばく露の発生源/構造
チャンバの詳細 exposure system was placed inside an air jacketed CO2 incubator
ばく露装置の詳細 exposure system consisted of a square-shaped Helmholtz coil, which was wound over a Teflon frame of dimension 35 x 35 x 20 cm (l x b x h); an uninterrupted power supply (UPS) provided less than 1% output voltage variation ensuring stable magnetic field generation; a step down transformer was used to reduce voltage down to 15 V, reducing the electric field component; the current source (step down transformer, rheostat etc.) was shielded by mild steel sheet enclosures
パラメータ
測定量 種別 Method Mass 備考
磁束密度 5 µT - 校正 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 for 16 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 50 µT - 校正 - -

ばく露3

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 for 16 hours
ばく露装置
ばく露の発生源/構造
  • E1と同じ装置
パラメータ
測定量 種別 Method Mass 備考
磁束密度 100 µT - 校正 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

A magnetic flux density dependent enhancement in the cell viability of lymphocytes was observed in exposure groups (groups 1-3) compared to the control group (group 4) with a significant difference between group 3 (100 µT exposure) and the control group. DNA laddering analysis showed a significant decrease in cell death in group 3 compared to the control group and flow cytometric analysis showed a significant decrease in apoptosis in group 2 (50 µT exposure) and group 3 compared to the control group. There was also a statistically significant decrease in the enzyme activity of caspase-9 in group 3 compared to the control group. Comet assay did not show any significant DNA damage in exposed cells but confirmed the decreased cell death in group 3 compared to the control group.
The authors conclude that exposure of sheep primary lymphocytes to a 50 Hz magnetic field could decrease apoptosis, possibly by the suppression of caspase-9 enzyme activity, leading to an enhanced cell viability.

研究の種別:

研究助成

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