Study type: Medical/biological study (experimental study)

Microarray gene expression profiling of a human glioblastoma cell line exposed in vitro to a 1.9 GHz pulse-modulated radiofrequency field. med./bio.

Published in: Radiat Res 2006; 165 (6): 636-644

Aim of study (acc. to author)

To assess the ability of exposure to a 1.9 GHz pulse modulated radiofrequency field to affect gene expression in glioblastoma cells.

Background/further details

Positive controls were performed at 43°C (1 h).

Endpoint

Exposure

Exposure Parameters
Exposure 1: 1.9 GHz
Modulation type: pulsed
Exposure duration: continuous for 4 h

Exposure 1

Main characteristics
Frequency 1.9 GHz
Type
Charakteristic
  • guided field
Polarization
  • circular
Exposure duration continuous for 4 h
Modulation
Modulation type pulsed
Duty cycle 33.3 %
Repetition frequency 50 Hz
Exposure setup
Exposure source
Chamber The temperature in the cell cultures was maintained at 37.0 ± 0.5°C by a mixture of prewarmed 95% air and 5% CO2.
Setup Cells were exposed in 10 ml of culture medium in 60-mm Petri dishes using a series of six circularly polarized cylindrical waveguides.
Sham exposure A sham exposure was conducted.
Additional info Negative and positive (heat shock) controls were run concurrently with each experiment.
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.1 W/kg mean measured - ±24%
SAR 1 W/kg mean measured - ±24%
SAR 10 W/kg mean measured - ±24%

Reference articles

  • Gajda GB et al. (2002): Cylindrical waveguide applicator for in vitro exposure of cell culture samples to 1.9-GHz radiofrequency fields.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

The data provided no evidence that non-thermal radiofrequency fields can affect gene expression in cultured U87MG cells in comparison to the control groups, whereas exposure to heat shock at 43°C up-regulated a number of typical stress-responsive genes in the positive control group.

Study character:

Study funded by

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