研究のタイプ: 医学/生物学の研究 (experimental study)

[ラット脳の坑酸化状態及び脂質レベルに対する超低周波電磁界急性ばく露の影響] med./bio.

Effect of acute extremely low frequency electromagnetic field exposure on the antioxidant status and lipid levels in rat brain.

掲載誌: Arch Med Res 2012; 43 (3): 183-189

この実験研究は、ラットの脳に対する60Hz磁界ばく露装置は直径30cmのヘルムホルツコイル。ケージを置いた空間の磁界強度2.4mT)の2時間急性ばく露の影響を調べた。Wistar雄ラット32匹を8匹ずつの4群に分けた(対照群拘束(MR)した対照群磁界ばく露群、磁界+MR群)。MRにはラット身体同等サイズのアクリル製シリンダーを用いた。ばく露後に屠殺して脳組織の分析を行った結果、磁界ばく露群ではカタラーゼスーパーオキシドディスムターゼの活性低下が生じた;磁界+MR群では還元グルタチオン一酸化窒素レベルが低下した、などの知見が得られたが、急性磁界ばく露ストレスホルモンであるコルチコステロンの上昇を引き起こさなかったと報告している。

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研究目的(著者による)

To study the immediate effects of acute magnetic field exposure, immobilization, and the combination of both on the antioxidant system and lipid content in the whole brain of rat.

詳細情報

32 male rats were divided into four groups: 1) control group, 2) exposure group, 3) immobilization, 4) exposure + immobilization.
Immobilization was used as a positive control for physical and psychological stress.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: continuous for 2 h

General information

Animals were divided into four groups: 1) control group (no exposure, no immobilization) 2) exposure group (no immobilization) 3) immobilization 4) exposure + immobilization

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 2 h
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 rats were restrained by confinement into a 18 cm long acrylic cylinder with an inner diameter of 7 cm; unrestained rats were placed individually in 47 cm x 25 cm x 21 cm acrylic cages; pair of circular Helmholtz coils with an inner diameter of 30 cm, consisting of 350 turns of 18-gauge copper wire; coils separated by 15 cm from the upper and lower surfaces of the animal cage
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 2.4 mT - 測定値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Acute exposure to extremely low frequency magnetic field alone as well as "exposure + immobilization" induced a reduction in catalase and superoxide dismutase enzyme activities compared with the control group, whereas the combination of "magnetic field exposure and immobilization" also decreased both reduced glutathione (compared with the control group) and nitric oxide levels compared with the other groups. Thiobarbituric acid reactive substances in the total lipid content of the brain were higher in the "exposure + immobilization group" than in the other experimental groups. Exposure to extremely low frequency magnetic fields did not induce any changes of the stress-hormone corticosterone or triacylglycerol levels.
Acute exposure to the extremely low frequency magnetic field appears to be a mild stressor.

研究の種別:

研究助成

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