Study type: Medical/biological study (experimental study)

Protective Effect of Caffeic Acid Phenethyl Ester (CAPE) on Liver and Kidney of Rats After Exposure to 900 MHz Electromagnetic Field. med./bio.

Published in: The Journal of American Science 2011; 7 (12): 937-944

Aim of study (acc. to author)

The effects of exposure of rats to a 900 MHz electromagnetic field on oxidative stress in the liver and kidney and the protective effect of CAPE should be investigated.

Background/further details

Rats were divided into 3 groups (n=16 each): 1) exposure to the electromagnetic field, 2) exposure to the electromagnetic field and CAPE administration, 3) sham exposed control group.
Animals in group 2 received daily intraperitoneal injections of 10 µM/ml/kg (?) caffeic acid phenethyl ester (CAPE) for 30 days before the daily exposure. The control group received sham injections of isotonic saline solution.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 900 MHz
Exposure duration: 1 hour/day for 60 days

Exposure 1

Main characteristics
Frequency 900 MHz
Type
Exposure duration 1 hour/day for 60 days
Exposure setup
Exposure source
  • monopole
Setup the exposure system was composed of a container designed in such a way that the animals were not restrained; the animals were left for a period to adapt and direct themselves spontaneously towards the antenna as the air opening was concentrated in the cover of the container around the antenna; the antenna (or two antennas set in parallel? contradictory statements) was placed at the center of the container
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
power density 0.02 mW/cm² - measured - -
SAR 1.075 W/kg mean calculated - -

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated system:
Time of investigation:
  • after exposure

Main outcome of study (acc. to author)

In the kidney, lipid peroxidation and nitric oxide were significantly increased and the enzyme activities of all antioxidative enzymes were significantly decreased in exposed rats (group 1) compared to the control group. Treatment with CAPE in addition to exposure (group 2) significantly reduced lipid peroxidation and nitric oxide and significantly increased the antioxidative enzyme activities compared to group 1. Histopathology revealed inflammatory reactions in the kidneys of group 1 and normal histology in groups 2 and 3.
In the liver, lipid peroxidation and nitric oxide were significantly increased and the enzyme activity of the glutathione peroxidase was significantly reduced in group 1 compared to the control group. Co-treatment with CAPE (group 2) significantly reduced lipid peroxidation and nitric oxide and significantly increased the glutathione peroxidase enzyme activity compared to group 1. However, Calatase and superoxide dismutase enzyme activities did not show any significant changes. Histopathology revealed signs of mild hepatitis in group 1 and normal histology in groups 2 and 3.
The authors conclude that exposure of rats to a 900 MHz electromagnetic field might induce oxidative stress in the liver and kidney and that CAPE might protect against these effects.

Study character:

Study funded by

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