研究のタイプ: 医学/生物学の研究 (experimental study)

[900 MHz電磁界ばく露後のラットの肝臓および腎臓に対するカフェイン酸フェネチルエステル(CAPE)の保護効果] med./bio.

Protective Effect of Caffeic Acid Phenethyl Ester (CAPE) on Liver and Kidney of Rats After Exposure to 900 MHz Electromagnetic Field.

掲載誌: The Journal of American Science 2011; 7 (12): 937-944

この研究は、900MHzの電磁界ばく露したラット肝臓での脂質過酸化(LPO)および抗酸化酵素の活性に対し、カフェイン酸フェネチルエステルによって生じるかも知れない防護作用を調査した。携帯電話電磁界は、主にLPOを増強すると見られるフリーラジカルを増加させ、酸化損傷につながるような肝臓の抗酸化活性を変化させることで、生体系に影響を及ぼすかも知れない。プロポリス抽出物の活性成分であるCAPEは抗酸化特性を示し、抗酸化物質の補充は電磁界ばく露による肝毒性に影響力を及ぼし得ることが、複数の研究で示唆されている。雄のSprague-Dawleyラット30匹を対照群(n=10)、900MHz電磁界ばく露群(n=10)、900MHz電磁界ばく露+CAPE投与群(n=10)に分けた。CAPEは電磁界ばく露の30日前に腹腔内注射した。肝組織を摘出し、カタラーゼ(CAT)、スーパーオキシドジスムターゼ(SOD)、グルタチオンペルオキシダーゼGSH-Px)、キサンチンオキシダーゼ(XO)の活性、およびLPOのレベルを調べた。その結果、対照群と比較して、900MHzばく露群ではXO、CATの活性、およびLPOのレベルが上昇したが、900MHzばく露+CAPE投与群ではXO、CATの活性およびLPOのレベルが低下した。対照群と比較して、900MHzばく露群ではSODおよびGSH-Pxの活性が低下したが、900MHzばく露+CAPE投与群ではこれらが上昇した。著者らは、CAPEは活性酸素種を減少させ、抗酸化酵素の活性を高めることで、抗酸化防御系を強め、900MHz電磁界による肝臓での酸化的変化を防ぐかも知れない、と結論付けている。

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研究目的(著者による)

The effects of exposure of rats to a 900 MHz electromagnetic field on oxidative stress in the liver and kidney and the protective effect of CAPE should be investigated.

詳細情報

Rats were divided into 3 groups (n=16 each): 1) exposure to the electromagnetic field, 2) exposure to the electromagnetic field and CAPE administration, 3) sham exposed control group.
Animals in group 2 received daily intraperitoneal injections of 10 µM/ml/kg (?) caffeic acid phenethyl ester (CAPE) for 30 days before the daily exposure. The control group received sham injections of isotonic saline solution.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
ばく露時間: 1 hour/day for 60 days

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 1 hour/day for 60 days
ばく露装置
ばく露の発生源/構造
  • モノポール
ばく露装置の詳細 the exposure system was composed of a container designed in such a way that the animals were not restrained; the animals were left for a period to adapt and direct themselves spontaneously towards the antenna as the air opening was concentrated in the cover of the container around the antenna; the antenna (or two antennas set in parallel? contradictory statements) was placed at the center of the container
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電力密度 0.02 mW/cm² - 測定値 - -
SAR 1.075 W/kg mean 計算値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In the kidney, lipid peroxidation and nitric oxide were significantly increased and the enzyme activities of all antioxidative enzymes were significantly decreased in exposed rats (group 1) compared to the control group. Treatment with CAPE in addition to exposure (group 2) significantly reduced lipid peroxidation and nitric oxide and significantly increased the antioxidative enzyme activities compared to group 1. Histopathology revealed inflammatory reactions in the kidneys of group 1 and normal histology in groups 2 and 3.
In the liver, lipid peroxidation and nitric oxide were significantly increased and the enzyme activity of the glutathione peroxidase was significantly reduced in group 1 compared to the control group. Co-treatment with CAPE (group 2) significantly reduced lipid peroxidation and nitric oxide and significantly increased the glutathione peroxidase enzyme activity compared to group 1. However, Calatase and superoxide dismutase enzyme activities did not show any significant changes. Histopathology revealed signs of mild hepatitis in group 1 and normal histology in groups 2 and 3.
The authors conclude that exposure of rats to a 900 MHz electromagnetic field might induce oxidative stress in the liver and kidney and that CAPE might protect against these effects.

研究の種別:

研究助成

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