Study type: Medical/biological study (experimental study)

Effect of 950 MHz UHF Electromagnetic radiation on biomarkers of oxidative damage, metabolism of UFA and antioxidants in the liver of young rats of different ages. med./bio.

Published in: Int J Radiat Biol 2014; 90 (2): 159-168

Aim of study (acc. to author)

To investigate the effects of an exposure to a 950 MHz electromagnetic field on biochemical processes in the liver of young rats.

Background/further details

During pregnancy, dams were exposed (n=6) or sham exposed (n=6). From each group, 6 pups were sacrificed in the age of 0, 6, 15 and 30 days, respectively and examined. In the age of 15 and 30 days, only male rats were examined.

Endpoint

Exposure

Exposure Parameters
Exposure 1: 950 MHz
Exposure duration: 30 minutes/day for 21 days during pregnancy
Exposure 2: 950 MHz
Exposure duration: 30 minutes/day for 21 days during pregnancy and postnatal until an age of 6 days
6 day-old rats
Exposure 3: 950 MHz
Exposure duration: 30 minutes/day for 21 days during pregnancy and postnatal until an age of 15 days
15 day-old rats
Exposure 4: 950 MHz
Exposure duration: 30 minutes/day for 21 days during pregnancy and postnatal until an age of 30 days
30 day-old rats

Exposure 1

Main characteristics
Frequency 950 MHz
Type
Exposure duration 30 minutes/day for 21 days during pregnancy
Additional info neonatal rats
Exposure setup
Exposure source
Distance between exposed object and exposure source 11 cm
Setup cages were placed inside an aluminium Faraday cage (101.5 x 64.0 x 38 cm); antenna was configured for vertical polarization
Sham exposure A sham exposure was conducted.
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.88 W/kg - calculated - for neonatal rats
SAR 0.01 W/kg minimum calculated - for pregnant rats
SAR 0.03 W/kg maximum calculated - for pregnant rats
electric field strength 28.9 V/m cf. remarks calculated - internal value for liver for neonatal rats
electric field strength 35 V/m mean measured - in the middle of the cage

Exposure 2

Main characteristics
Frequency 950 MHz
Type
Exposure duration 30 minutes/day for 21 days during pregnancy and postnatal until an age of 6 days
Additional info 6 day-old rats
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.51 W/kg - calculated - for 6 day-old rats
SAR 0.01 W/kg minimum calculated - for pregnant rats
SAR 0.03 W/kg maximum calculated - for pregnant rats
electric field strength 22 V/m cf. remarks calculated - internal value for liver for 6 day-old rats
electric field strength 35 V/m mean measured - in the middle of the cage

Exposure 3

Main characteristics
Frequency 950 MHz
Type
Exposure duration 30 minutes/day for 21 days during pregnancy and postnatal until an age of 15 days
Additional info 15 day-old rats
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.18 W/kg - calculated - for 15 day-old rats
SAR 0.01 W/kg minimum calculated - for pregnant rats
SAR 0.03 W/kg maximum calculated - for pregnant rats
electric field strength 15.7 V/m cf. remarks calculated - internal value for liver for 15 day-old rats
electric field strength 35 V/m mean measured - in the middle of the cage

Exposure 4

Main characteristics
Frequency 950 MHz
Type
Exposure duration 30 minutes/day for 21 days during pregnancy and postnatal until an age of 30 days
Additional info 30 day-old rats
Exposure setup
Exposure source
Parameters
Measurand Value Type Method Mass Remarks
SAR 0.06 W/kg - calculated - for 30 day-old rats
SAR 0.01 W/kg minimum calculated - for pregnant rats
SAR 0.03 W/kg maximum calculated - for pregnant rats
electric field strength 8.5 V/m cf. remarks calculated - internal value for liver for 30 day-old rats
electric field strength 35 V/m mean measured - in the middle of the cage

Reference articles

  • Vecchia P et al. (2009): Exposure to high frequency electromagnetic fields, biological effects and health consequences (100 kHz-300 GHz).
  • Peyman A et al. (2001): Changes in the dielectric properties of rat tissue as a function of age at microwave frequencies.

Exposed system:

Methods Endpoint/measurement parameters/methodology

Investigated material:
Time of investigation:
  • during exposure
  • after exposure

Main outcome of study (acc. to author)

Neonatal rats exposed in utero had a significanty decreased body weight, significantly decreased levels of catalase and significant lower lipid peroxidation values compared to neonatal rats from the control group. No significant differences in the body weight, the levels of catalase and the lipid peroxidation were observed after 6, 15 or 30 days between the groups.
No differences occurred in the level of protein oxidation between exposed and sham exposed rats.
The level of fatty acids was significantly decreased in exposed rats in comparison to sham exposed rats (Remark EMF-Portal: information about age of rats not given in this context).
15 and 30 day-old rats showed significant differences regarding DNA damage. In the age of 15 days, in the exposed rats, the number of DNA strand breaks was significantly lower compared to the control group, while it was significantly higher in the age of 30 days.
The data indicate that exposure to 950 MHz electromagnetic fields could have induced some biochemical changes in the liver of young rats.

Study character:

Study funded by

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