研究のタイプ: 医学/生物学の研究 (experimental study)

[900 MHz変調ラジオ周波数電磁界にばく露された神経芽様細胞株の増殖とアポトーシス] med./bio.

Proliferation and apoptosis in a neuroblastoma cell line exposed to 900 MHz modulated radiofrequency field.

掲載誌: Bioelectromagnetics 2006; 27 (3): 164-171

この研究は、国際非電離放射線防護委員会ICNIRP)の職業ばく露参考レベルより高い SARレベルの、携帯電話変調無線周波RF電磁界が、神経芽細胞腫細胞株増殖分化、およびアポトーシスプロセスに変化を引き起こすことができるか調べた。同期細胞培養指数関数的増殖期の細胞株に、900 MHzの24、48、72時間ばく露を与え、増殖および分化をWST-Iアッセイおよび特定のマーカ(2つの発がん遺伝子および細胞骨格タンパク質)の分子分析により評価した。アポトーシスは、カスパーゼ活性化分析およびポリ(ADP-リボース)ポリメラーゼPARP)切断分子検出により評価した。RFとの組み合わせばく露には分化誘導剤レチノイン酸またはアポトーシス誘導剤カンプトテシンを用い、その組み合わせばく露によって電磁界化学物質との間の起こり得る干渉を試験した。結果として、全般的に、最大72時間の900 MHzの高周波ばく露は、神経芽細胞腫細胞株の3つの主要な細胞活動に大きな変化を引き起こさないことが示された、と報告している。

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研究目的(著者による)

To study whether a modulated radiofrequency of the type used in cellular phone communications at a specific absorption rate higher than International Commission on Non-Ionizing Radiation Protection (ICNIRP) reference level for occupational exposure, could elicit alterations on the three principal cellular processes as cell proliferation, cell differentiation, and apoptosis processes in a neuroblastoma cell line.

詳細情報

Combined exposures to radiofrequency and to the differentiative agent retinoic acid or to the apoptotic inducer camptothecin were carried out to study possible interaction between electromagnetic field and chemical agents.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 24, 48 and 72 h
  • SAR: 1 W/kg mean

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 24, 48 and 72 h
Additional information Reference article on thermal power pulse method: Hand JW, Lagendijk JW, Bach Andersen J, Bolomey JC. 1989. Quality Assurance Guidelines for ESHO Protocols. Int J Hyperthermia 5:421-428. (publication 14294 )
Modulation
Modulation type pulsed
Additional information

GSM signal

ばく露装置
ばく露の発生源/構造
  • Wire Patch Cells (WPC)
チャンバの詳細 Four WPCs were placed into two incubators (one for RF and one for sham exposure using blind procedure). Each WPC was shielded by a 40 x 40 x 20 cm metal grid box and four blocks of foam absorber material (15 dB). The absence of any interference between the two WPCs located in the same incubator was confirmed by measurement.
ばく露装置の詳細 The WPCs consisted of two parallel plates short-circuited at the edge at four points [Laval et al., 2002]. Four Petri dishes (3.5 cm in diameter) were placed in each WPC, one of them filled only with medium for monitoring temperature. A water spiral plate jacket was positioned on the external faces of both plates of the WPC with circulating water maintaining a temperature of 37 °C in the medium.
Sham exposure A sham exposure was conducted.
Additional information As a further control, the same number of dishes were placed in another incubator without exposure system.
パラメータ
測定量 種別 Method Mass 備考
SAR 1 W/kg mean 測定値および計算値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The data suggest that 900 MHz radiofrequency exposure up to 72 h does not induce significant alterations in the three principal cell activities in a neuroblastoma cell line.

研究の種別:

研究助成

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