研究のタイプ: 医学／生物学の研究 (experimental study)

[MCF-7細胞での遺伝子およびタンパク質発現に対するGSM1800MHzの無線周波電磁界の影響] med./bio.

Effects of global system for mobile communications 1800 MHz radiofrequency electromagnetic fields on gene and protein expression in MCF-7 cells

著者: Zeng Q, Chen G, Weng Y, Wang L, Chiang H, Lu D, Xu Z
掲載誌: Proteomics 2006; 6 (17): 4732-4738
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この研究は、ゲノム解析およびプロテオーム解析の手法を用いて、1800 MHz GSM携帯電話信号を模擬した高周波電磁界（RF EMF）に対するヒト乳がん細胞株MCF-7の反応を、網羅的に遺伝子およびタンパク質を解析して評価した。RF EMFばく露は、最大3.5 W / kgまでのSARで３時間行われた。その結果、GeneChip分析により、ばく露後に一貫性のある差分的変化を示した少数の遺伝子が特定された；しかし、これらの遺伝子について、その後に実施したリアルタイムPCRアッセイではより詳細な情報は確認できなかった；一方、MCF-7細胞の体系的なプロテオーム解析では、SARが3.5 W / kgでの24時間以内の連続または断続的なRF EMFばく露により、少数の、前述とは別のタンパク質の差分的発現が示された；これらの知見は、観察された影響が偶然によるものである可能性を示唆する、と報告している。

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研究目的（著者による）

To study the effects of radiofrequency electromagnetic field exposure on gene and protein expression profiles in human breast cancer cell line MCF-7.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,800 MHz
Modulation type: pulsed
ばく露時間: intermittent, 5 min on/10 min off, for 24 h
transcriptome analysis
  • SAR: 2 W/kg mean
  • SAR: 3.5 W/kg mean
ばく露2: 1,800 MHz
Modulation type: pulsed
ばく露時間: intermittent, 5 min on/10 min off, for 1, 3, 6, 12, and 24 h
proteome analysis
  • SAR: 3.5 W/kg mean
ばく露3: 1,800 MHz
Modulation type: pulsed
ばく露時間: continuous for 1, 3, 6, 12, and 24 h
proteome analysis
  • SAR: 3.5 W/kg mean

General information

The computer controlled exposure system has been described in detail by the designer and other groups [Schönborn et al., 2001 and Diem et al., 2005].

ばく露1

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 intermittent, 5 min on/10 min off, for 24 h
Additional information transcriptome analysis
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
Additional information

GSM signal
ばく露装置
ばく露の発生源／構造
チャンバの詳細 Two waveguides, one for RF and one for sham exposure, were placed inside a conventional incubator at 37°C, 5% CO2 and 95% air.
ばく露装置の詳細 Six 35-mm Petri dishes were placed in each waveguide in a dish holder holding them in the H-field maximum of the standing wave and exposed simultaneously in E polarization. The system enabled the exposure of monolayers of cells with a SAR non-uniformity of less than 30%.
Sham exposure A sham exposure was conducted.
Additional information The temperature of the system remained at 37 ± 0.1°C during the whole exposure duration, and the temperature difference between RF and sham exposed cultures never exceeded 0.1°C.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg mean - - -
SAR 3.5 W/kg mean - - -

ばく露2

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 intermittent, 5 min on/10 min off, for 1, 3, 6, 12, and 24 h
Additional information proteome analysis
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
Additional information

GSM signal
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 3.5 W/kg mean - - -

ばく露3

主たる特性
周波数 1,800 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 1, 3, 6, 12, and 24 h
Additional information proteome analysis
Modulation
Modulation type pulsed
Duty cycle 12.5 %
Repetition frequency 217 Hz
Pulse type rectangular
Additional information

GSM signal
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 3.5 W/kg mean - - -

Reference articles

  • Diem E et al. (2005): [イン・ビトロでのヒト線維芽細胞および形質転換GFSH-R17ラット顆粒膜細胞における携帯電話放射（1800MHz）による非熱的なDNA切断]
  • Schönborn F et al. (2001): [移動通信の健康ハザード評価にためのIn vitroばく露装置の最適化]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

DNA microarray analysis identified a handful of consistent changed genes after exposure to radiofrequency electromagnetic field at specific absorption rates of up to 3.5 W/kg for 24 h. However, these differentially transcribed genes could not be further confirmed by RT-PCR assay.
Meanwhile, systematic proteome analysis revealed that a few but different proteins were differentially expressed under continuous or intermittent radiofrequency electromagnetic field exposure at SAR of 3.5 W/kg for 24 h or less, implying that the observed effects might have occurred by chance.
In conclusion, the data do not provide convincing evidence that radiofrequency electromagnetic field exposure under the present experimental conditions can produce distinct effects on gene and protein expression in the MCF-7 cells.

研究の種別:

研究助成

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