研究のタイプ: 医学/生物学の研究 (experimental study)

[マウスの精巣組織に対するUHV送電線の電磁界ばく露の影響] med./bio.

Effects of Electromagnetic Field of UHV Transmission Lines Exposure on Testis Tissue in Mice

掲載誌: IEEE Trans Magn 2009; 45 (10): 4853-4856

この研究の目的は、マウスの精巣組織照射された送電線電磁界((ELF)の推定される影響を評価することであった。40匹の成体BALB/cマウスが2つのグループに分けられた。1つのグループ(20匹)は1日24時間、毎日のばく露が行われ、他の擬似ばく露グループ(20匹)は実験対照として扱われた。7週間のばく露後、2つのグループのマウスが犠牲にされた。精巣組織は、血清生化学的分析、組織病理学的検査、およびフローサイトメトリ-分析(FCM)によって評価された。電磁界ばく露は、DNA倍数性 (1n、2n、4n細胞)の分布にそれほど影響しなかったが、精巣生殖細胞のS期分画および増殖指数を著しく減少させた。

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研究目的(著者による)

To investigate whether extremely low frequency magnetic fields similar to those of transmission lines affect the testis tissue of mice.

詳細情報

40 mice (20 male, 20 female) were divided into an exposure and a sham exposure group (10 males and 10 females in each group).
Remark EMF-Portal: The employment of female mice does not make sense in the examined endpoints (testis).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 7 weeks (24 h/day)

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • electric field
  • magnetic field
ばく露時間 continuous for 7 weeks (24 h/day)
ばく露装置
ばく露の発生源/構造
  • power line set-up
チャンバの詳細 5 mice were kept in one cage, respectively
ばく露装置の詳細 electric field was induced by three-phase voltage lines which were connected to a secondary coil of the step-up transformer; lines were assumed to be straight, parallel to each other, with an equal distance (0.3 m); magnetic field was induced by three-phase current lines connected to a secondary coil of the step-down transformer and located beneath the insulating board; ground clearance of central line of the insulating board was 0.45 meter; primary coils of two transformers were respectively connected to two three-phase voltage dividers; mice cages were fixed at one side of B phase line (mid line)
パラメータ
測定量 種別 Method Mass 備考
電界強度 4.5 kV/m maximum 計算値 - -
電界強度 3.5 kV/m minimum 計算値 - -
磁束密度 40 µT minimum - - -
磁束密度 48 µT maximum - - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

No significant differences in the serum level of gamma-glutamyltransferase were found between the exposure and the sham exposure group (remark EMF-Portal: no data given for the level of gamma-glutamyltransferase in the testis).
The histopathological examination of the testis revealed no differences between the exposure and the sham exposure group.
In exposed rats, the cell proliferation and the number of germ cells in the S-phase were significantly decreased compared to the sham exposed rats, while no difference regarding the number of chromosome sets was observed.

研究の種別:

研究助成

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