研究のタイプ: 医学/生物学の研究 (experimental study)

[超低周波磁界にばく露した新生ラットの脳と骨髄の細胞における遺伝毒性および細胞毒性ハザードの評価] med./bio.

Assessment of genotoxic and cytotoxic hazards in brain and bone marrow cells of newborn rats exposed to extremely low-frequency magnetic field.

掲載誌: J Biomed Biotechnol 2012: 716023

この研究は、新生ラットの脳と骨髄細胞における遺伝毒性および細胞毒性に対する超低周波磁界全身ばく露の影響を調べた。生後10日目のラットに、50Hz、0.5mTの磁界を24時間、30日連続ばく露した。ばく露後、脳と骨髄を摘出し、検査した。ばく露群、対照群は各10匹。その結果、ばく露群で見られた有意な影響は、DNA損傷を示すコメットアッセイでのテールモーメント平均値の増加、小核誘導および細胞分裂指数の上昇、マロンジアルデヒドとスーパーオキシジムスターゼのレベル上昇であり、グルタチオン活性は変化しなかった、と報告している。

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研究目的(著者による)

To examine the cytotoxic and genotoxic effects and the level of oxidative stress in the brain and bone marrow cells after an exposure to extremely low frequency magnetic fields of newborn rats.

詳細情報

Exposure or sham exposure started at an age of 10 days and lasted for 30 days. In total, 60 rats were examined. For the micronucleus test, comet assay and the histopathological examination, 10 rats per group (exposed/sham exposed) were used. Another 10 rats per group were used for the determination of the mitotic index and the last 10 rats per group for the evaluation of the oxidative stress.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 24 h/day on 30 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 24 h/day on 30 days
ばく露装置
ばく露の発生源/構造
チャンバの詳細 40 cm x 25 cm x 20 cm plastic cage housing 30 young rats
ばく露装置の詳細 solenoid constructed by winding 320 turns of insulated 0.8 mm copper wire round a 2 mm thick, 40 cm long copper cylinder with a diameter of 40 cm; cylinder earthed; cage placed in the center of the solenoid
Sham exposure A sham exposure was conducted.
Additional information field was switched off during cage cleaning
パラメータ
測定量 種別 Method Mass 備考
磁束密度 0.5 mT - 測定値 - ± 0.025 mT

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

In the exposed group, the enzyme activity of the superoxide dismutase and the lipid peroxidation were significantly increased compared to the sham exposed group. In the histopathological examination of the brain tissue, only slight differences were found between the sham exposed and exposed rats (mild degeneration in the glia, gliosis, vascular congestion).The DNA damage in the brain, as well as the mitotic index and the chromosome damage in the bone marrow cells were significantly increased in the exposed rats compared to the sham exposed rats.
The authors conclude that exposure to extremely low frequency magnetic fields could lead to cytotoxic and genotoxic damage, as well as to oxidative stress in the brain and bone marrow of newborn rats.

研究の種別:

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