研究のタイプ: 医学/生物学の研究 (experimental study)

[超低周波電磁界はイン・ビトロの胚性神経幹細胞においてTRPC1増加を介して神経分化と神経突起伸長を促進する] med./bio.

Extremely low-frequency electromagnetic fields promote in vitro neuronal differentiation and neurite outgrowth of embryonic neural stem cells via up-regulating TRPC1.

掲載誌: PLoS One 2016; 11 (3): e0150923

この研究は、胚性神経幹細胞(eNSCs)に対する超低周波磁界ばく露(ELF-MF:50 Hz、1 mT;1日4時間ばく露を1、2、3日間)の影響を調べた。その結果、増殖培養液中でのELF-MFばく露後、eNSCの増殖とその維持は有意に増強した;また、ELF-MFばく露後に、分化した神経細胞の比率の上昇および神経突起伸長の促進も観察された、などのいくつかの所見を報告している。

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研究目的(著者による)

The effects of exposure of mouse embryonic neural stem cells to a 50 Hz magnetic field on neuronal differentiation and development and the underlying molecular mechanisms of action should be investigated.

詳細情報

To investigate the role of TRPC1 ion channels, the TRPC1 translation was inhibited (a so called gene knockdown) via transfection with TRPC1-siRNA (small interfering RNA). TRPC1 is a calcium ion channel and the intracellular calcium level is believed to be associated with cell differentiation.
Results were obtained from five independent duplicate experiments.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 4 h/day for 1, 2 or 3 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 4 h/day for 1, 2 or 3 days
ばく露装置
ばく露の発生源/構造
  • 詳細不明
チャンバの詳細 two identical chambers placed inside a commercial incubator
ばく露装置の詳細 environmental conditions in chambers were kept stable (37°C, 5% CO2, 95% humidity); the temperature variance between the chambers did not exceed 0.3°C; during exposure, the chambers were randomly assigned to sham exposure or exposure by a computer program
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - - - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Cell viability, cell maintenance, cell proliferation and gene expression of proliferation-related genes were significantly increased after exposure for 3 days compared to sham exposed cells.
Differentiation into neurons was significantly increased in exposed cells compared to sham exposed cells after 3 days, as shown by immunohistochemical stain, protein expression and gene expression of neuronal markers (NeuroD and Ngn1). Moreover, the length of neurites and the number of branch points was significantly increased in exposed cells in comparison to sham exposed cells.
Exposure to the magnetic field had no effect on apoptosis.
The magnetic field exposure led to a significantly increased TRPC1 gene expression and protein expression (no differences were observed for TRPC3-7) and to a significantly increased intracellular calcium peak level after 3 days compared to the sham exposure. However, in cells with TRPC1 knockdown, the effects of the magnetic field on neuronal differentiation and the calcium level were reversed.
The authors conclude that exposure of mouse embryonic neural stem cells to a 50 Hz magnetic field might stimulate proliferation and neuronal differentiation via upregulation of the expression of TRPC1 and neuronal genes (NeuroD and Ngn1).

研究の種別:

研究助成

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