この研究は、胎仔の脳および成獣の虚血脳から採取し培養した海馬の神経前駆細胞（NPCs）に対する超低周波磁界（ELF-EMF：50 Hz、0.4 mT）の影響を調べた。その結果、胎仔由来NPCsおよび虚血脳由来NPCsの両方において、ELF-EMFばく露による増殖力の増強が見られた；累積的なELF-EMFばく露後7日目において、神経細胞の分化は増強されたが、神経膠細胞の分化には顕著な影響はなかった；虚血脳由来NPCsでは、ELF-EMFばく露による増殖中にリン酸化Aktの発現が増えたが、Akt経路を遮断すると、虚血脳由来NPCsでのELF-EMF誘導性の増殖は見られなくなった､と報告している。
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The effects of exposure of neural progenitor cells from adult ischemic and embryonic mice brains to a 50 Hz magnetic field on proliferation and neural differentiation should be investigated.
Neural progenitor cells play a crucial role in regeneration after brain injuries and may therefore be of therapeutic use.
Cells were divided into the following groups: 1) magnetic field exposure of embryo-derived cells, 2) control group for embryo-derived cells, 3) magnetic field exposure of adult-derived cells, 4) co-exposure of adult-derived cells to magnetic field and Wortmannin, 5) control group for adult-derived cells. Wortmannin is a specific inhibitor of the protein kinase B, whose signal pathway was investigated as a possible underlying mechanism of action.
ばく露時間: continuous for up to 7 days
|ばく露時間||continuous for up to 7 days|
|ばく露装置の詳細||the solenoid was positioned into a water-jacketed temperature- and atmosphere-regulated incubator (37 ± 0.4°C, 5% CO2)|
|Additional information||control samples were placed in another identical incubator|
|磁束密度||0.4 mT||-||測定値||-||uniformity of field ± 0.012 mT|
Exposure to the magnetic field significantly increased the cell proliferation and the amount of differentiated neurons in both embryo-derived (group 1) and adult-derived (group 3) cells compared to their respective control group (group 2 or 5). Astrocyte differentiation did not show any significant differences between the exposure and control groups. The oligodendrocyte differentiation could not be analyzed due to a lack of detectable markers.
The amount of phosphorylated protein kinase B was significantly increased in exposed adult-derived cells (group 3) in comparison to the respective control group. Co-exposure to magnetic field and Wortmannin, however, decreased the amount of phosphorylated protein kinase B and reduced the cell proliferation significantly compared to the exposure group. All other signal pathway markers did not show any significant differences between the groups.
The authors conclude that exposure of neural progenitor cells from adult ischemic and embryonic mice brains to a 50 Hz magnetic field might enhance proliferation and neuronal differentiation. The effects of the magnetic field might be mediated via the protein kinase B pathway.