この研究は、毛包の真皮乳頭細胞（DPCs）でのMAP2およびネスチン遺伝子の発現に対する超低周波電磁界（ELF-EMF：50 Hz, 1 mT）の影響を調べた。4つの実験条件（ELF-EMF単独ばく露、化学的因子（薬剤名は不記載）単独ばく露、化学的因子＋ELF-EMFばく露、対照（無処置））で、それぞれ5日間ばく露を行った。また、EMF単独ばく露についてはばく露期間を14日間に延長もした。その結果、EMFの5日間ばく露に比べ、14日間のばく露において、MAP2発現が増加し、細胞増殖が低下した、とのみ報告している。
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The effects of exposure of dermal papilla mesenchymal cells to a pulsed 50 Hz magnetic field on the gene expression of neural differentiation markers should be investigated.
Cells were divided into the following groups: 1) exposure to magnetic field for 5, 14 and 20 days, 2) treatment with neural differentiation medium for 5 days, 3) co-exposure to the magnetic field and neural differentiation medium for 5 days and 4) control group.
|Pulse width||40 ms|
|Duty cycle||60 %|
|チャンバの詳細||solenoid inside an incubator with 5% CO2 and 37°C|
|ばく露装置の詳細||samples were placed at the center of a uniform field area inside a multi-turn solenoid|
The gene expression of MAP2 was significantly increased in cells exposed to the magnetic field (group 1) for 14 days and in cells treated with neural differentiation medium (group 2), with group 2 showing the most distinct increase. However, no MAP2 expression was found in cells co-exposed to the magnetic field and differentiation medium (group 3). The Nestin gene expression was significantly increased in cells from group 1 after 14 days and group 3, with the most distinct increase in group 3.
In morphological investigations, cells from group 1 showed the unipolar and multipolar morphology of neurons after 5 or 14 days, while in group 2, a large number of dead cells was observed.
Cell proliferation was decreased in group 1 after 5, 14 and 20 days compared to the control group (significance not stated), supporting an increased differentiation in this group.
The authors conclude that exposure of dermal papilla mesenchymal cells to a pulsed 50 Hz magnetic field might increase the gene expression of the neural differentiation markers Nestin and MAP2.