研究のタイプ: 医学/生物学の研究 (experimental study)

[超低周波電磁界は骨髄由来間葉系幹細胞の神経分化を誘導する] med./bio.

Extremely low-frequency electromagnetic fields induce neural differentiation in bone marrow derived mesenchymal stem cells.

掲載誌: Exp Biol Med (Maywood) 2013; 238 (8): 923-931

この研究は、超低周波電磁界(ELF-EMF:50Hz、1mT)が骨髄由来間葉幹細胞(BM-MSCs)の神経分化誘導するか否かをインビトロ実験した。BM-MSCsをELF-EMFばく露下で12日間培養し、ELF-EMFのばく露なしで培養した対照群と比較した。その結果、ばく露群では、増殖速度が有意に低下し、反対に神経分化の増加が見られた;神経分化マーカ(MAP2)のレベルが上昇し、早期神経マーカ(ネスチン)は下方調節された、などを報告している。

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研究目的(著者による)

To examine the beneficial effects of an exposure to extremely low frequency magnetic fields on the protein expression during cell differentiation in bone marrow derived mesenchymal stem cells.

詳細情報

Bone marrow derived mesenchymal stem cells have the potential to differentiate into nerve type cells.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for up to 12 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for up to 12 days
ばく露装置
チャンバの詳細 cells were incubated in an incubator at 37 °C and 5 % CO2
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT - - - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

The cell number of the exposed cell cultures was significantly decreased after 6 and 12 days compared to the control cell culture. Additionally, the cells from the exposed groups showed morphological changes that resembled neurite-like features: They appeared to be narrow, elongated and more branched than cells from the control cultures. In exposed cells, the differentiation marker "microtubule associated protein 2" was significantly increased in comparison to the control cells, while the level of the early neural marker nestin was significantly decreased. The intracellular level of calcium was significantly increased in the exposed cell cultures compared to the control cell cultures.
Using two-dimensional gel electrophoresis, 8 spots with a difference in the protein expression were detected and identified with mass spectrometry. Only the protein expression of ferritin was verified via Western blot.
The data indicate that exposure to extremely low frequency magnetic fields promotes cell differentiation in bone marrow derived mesenchymal stem cells and may help to understand the effect of extremely low frequency magnetic field stimulation on bone marrow derived mesenchymal stem cells during neural differentiation and its potential use as a clinically therapeutic option for treating neurodegenerative diseases.

研究の種別:

研究助成

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