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To examine the beneficial effects of an exposure to extremely low frequency magnetic fields on the protein expression during cell differentiation in bone marrow derived mesenchymal stem cells.
Bone marrow derived mesenchymal stem cells have the potential to differentiate into nerve type cells.
ばく露時間: continuous for up to 12 days
The cell number of the exposed cell cultures was significantly decreased after 6 and 12 days compared to the control cell culture. Additionally, the cells from the exposed groups showed morphological changes that resembled neurite-like features: They appeared to be narrow, elongated and more branched than cells from the control cultures. In exposed cells, the differentiation marker "microtubule associated protein 2" was significantly increased in comparison to the control cells, while the level of the early neural marker nestin was significantly decreased. The intracellular level of calcium was significantly increased in the exposed cell cultures compared to the control cell cultures.
Using two-dimensional gel electrophoresis, 8 spots with a difference in the protein expression were detected and identified with mass spectrometry. Only the protein expression of ferritin was verified via Western blot.
The data indicate that exposure to extremely low frequency magnetic fields promotes cell differentiation in bone marrow derived mesenchymal stem cells and may help to understand the effect of extremely low frequency magnetic field stimulation on bone marrow derived mesenchymal stem cells during neural differentiation and its potential use as a clinically therapeutic option for treating neurodegenerative diseases.