研究のタイプ: 医学/生物学の研究 (experimental study)

[PC12細胞のNGF誘導性神経分化に対する超低周波磁界の影響] med./bio.

Effects of extremely low frequency magnetic fields on NGF induced neuronal differentiation of PC12 cells.

掲載誌: Bioelectromagnetics 2014; 35 (7): 459-469

この研究は、PC12細胞の神経増殖因子NGF誘導神経分化に対する超低周波磁界ばく露(ELF-MFs:50 Hz、1 mT、5日間)の影響をプロテオミック手法で調べた。その結果、ばく露群では、神経突起の長さ、神経突起を持つ細胞の数が増加し、増殖活性は低下した;NGFのみの群とNGF+ ELF-MFs群で、発現するたんぱく質に違いが見られた、と報告している。

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研究目的(著者による)

To investigate the effects of extremely low frequency magnetic fields on the neuronal differentiation of PC-12 cells.

詳細情報

In this study, PC-12 cells were used as a model to examine neural differentiation. These cells differentiate and change their morphology into neuron-like cells by stimulation with the nerve growth factor. A better understanding of neural differentiation, including proteomic analyses, may lead to new therapeutic options in the treatment of neurodegenerative diseases.
To induce neuronal differentiation of the cells, nerve growth factor was added to the media at a concentration of 50 ng/ml. The following groups were examined: 1.) control group, 2.) addition of nerve growth factor and 3.) addition of nerve growth factor + magnetic field exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 1, 3, or 5 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 1, 3, or 5 days
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 two coils (17.5 cm radius each) arranged in a Helmholtz configuration oriented to produce a vertical magnetic field; distance between the coils 17.5 cm; number of loops 1000
パラメータ
測定量 種別 Method Mass 備考
磁束密度 1 mT effective value 測定値 - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

Results of the magnetic field exposure group treated with nerve growth factor (group 3) were compared to the results of the group treated with nerve growth factor alone (group 2).
After 5 days, magnetic field exposed cell culture had significantly longer neurite outgrowths and more neurite bearing cells, while the cell proliferation was significantly decreased, which is associated with an enhancement in differentiation.
A magnetic field exposure significantly altered the gene expression and protein expression related to differentiation. The two-dimensional gel electrophoresis showed a different protein expression in magnetic field exposed cell cultures. Therefore, six of these differentially expressed proteins were identified via mass spectrometry. The identified proteins are known to be involved in cell differentiation processes or are associated with human brain disorders (e.g. neurodegenerative diseases).
The authors conclude that exposure to extremely low frequency magnetic fields promoted the differentiation in PC-12 cells and that these fields might represent a potential therapeutic option in the treatment of neurodegenerative diseases.

研究の種別:

研究助成

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