研究のタイプ: 医学/生物学の研究 (experimental study)

[50Hz電磁界はラット骨の間葉系間質細胞の機能的ニューロンへの分化誘導を促進する] med./bio.

Fifty-Hertz electromagnetic fields facilitate the induction of rat bone mesenchymal stromal cells to differentiate into functional neurons.

掲載誌: Cytotherapy 2013; 15 (8): 961-970

この研究は、インビトロでのラットの骨の間葉間質細胞(BMSC)の神経細胞への分化誘導に対する電磁界EMF)の有効性を調べた。SDラットからBMSCを所定の方法で単離し、培養後、所定の密度で細胞を播いた培養皿を3群(分化誘導剤+EMF群、分化誘導剤群、無処置の対照群)準備した。EMFは、50Hz、5mTの磁界で、1日60分間、12日間ばく露した。全ての処置終了後、共焦点顕微鏡による細胞の表現型の観察、RT-PCRによる遺伝子発現の測定、細胞のパッチクランプ法によるシナプス電流の検出、フローサイトメトリーによる細胞周期神経細胞マーカが表れる細胞の割合の分析を行った。その結果、EMF処置群では、BMSCの神経細胞への分化促進された;神経細胞分化した細胞シナプス結合を形成し、興奮性のシナプス電流を発射した;EMF処置群では、G0-G1期の細胞の割合が徐々に低下し、S期の割合および神経細胞マーカが表れる細胞の割合が増加した、と報告している。

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研究目的(著者による)

To investigate the influence of 50 Hz-magnetic fields on the differentiation of rat bone mesenchymal stromal cells into neurons.

詳細情報

Three groups of cell cultures were examined: 1.) induction + magnetic field exposure, 2.) induction and 3.) control group (neither exposure nor induction).
For induction, cell cultures were treated with growth factors.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: 60 min/day for up to 12 days (or 30 min? Contradictory information)

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 60 min/day for up to 12 days (or 30 min? Contradictory information)
ばく露装置
ばく露の発生源/構造
ばく露装置の詳細 dimensions of coils: 40 mm high, 100 mm long, 100 mm inner diameter, 200 mm outer diameter; 200 turns with a radius of 70 mm
パラメータ
測定量 種別 Method Mass 備考
磁束密度 5 mT - 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム(著者による)

The morphology of the cells changed during the 10 days of observation into neuron-like cells with functional neural electrophysiological activity. Particularly in the exposed group, a high number of neuron-like cells was found when compared to the other groups. Exposed cells showed significantly increased surface protein expression of neuron-specific-enolase compared to the induction group, which indicates a more mature stage. The percentage of cells in the G0 phase and G1 phase was significantly decreased in the exposed group as compared to the other groups, while the percentage of cells in the S-phase was significantly increased. Gene expression of beta-tubulin III, glial fibrillary acidic protein and brain-derived neurotrophic factor was highest in the exposure group and lowest in the control group, while in the induction group, gene expression of these genes was higher than in the control group but lower in comparison to the exposure group. In neither of the groups, gene expression of nestin was observed.
Taken together, the data indicate that exposure to 50 Hz-magnetic fields could promote the differentiation of rate bone mesenchymal stromal cells into functional neurons. Therefore, according to the authors, extremely low frequency magnetic fields could represent a potential therapeutic option for treating neurodegenerative diseases.

研究の種別:

研究助成

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