＜目的＞携帯電話から発生する変調されたラジオ波が、様々なストレス応答を引き起こすかどうかを調べる。 ＜方法＞増殖期、定常期の初期、定常期のマウスの細胞に4日間ラジオ波を照射した。それらの細胞からRNA抽出し、RT-PCRを行い、fos、jun、mycのmRNAの発現レベルを調べた。また、タンパクを抽出し、Gel MobilityShift Assayを行ってAP-1、AP-2、NF-kapperBのDNA結合活性を調べた。さらに血清刺激の影響も調べた。 ＜結果＞jun、mycのmRNAの発現レベルは、曝露開始時の細胞増殖の段階に関係無く変化しなかった。また、DNA結合活性はいずれも変化しなかった。しかし、fosだけはほとんどの条件で増加した。
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To determine whether two different modulated radiofrequencies of the type generally used in cellular phones (835.62 MHz (FMCW) and 835.62 MHz (CDMA)) could elicit a general stress response in a biological system.
Changes in proto-oncogene expression, determined by measuring Fos, Jun, and Myc mRNA levels as well as by the DNA-binding activity of the AP1, AP2 and NFkB transcription factors, were used as indicators of a general stress response.
|ばく露時間||continuous for 24 h or 4 days|
|Modulation type||cf. additional information|
|チャンバの詳細||A detailed description of the radial transmission line (RTL) and a temperature-controlled facility containing nine RTLs has been given in the reference articles. The RTL is a shielded irradiator consisting of a parallel-plate region (43 mm spacing, total radius of 533 mm) with the bottom aluminium plate acting as a thermal homogenizer for the flasks. A central conical antenna (203 mm in diameter) emitted TEM waves radially outward that were terminated by RF-absorbing material backed by a lamina of perforated aluminium.|
|ばく露装置の詳細||A total of 16 T-75 culture flasks each containing 40 ml of medium could be distributed angularly around the antenna. The cell layer in the flasks was subjected to a perpendicular EF and a circumferential MF perpendicular to the EF and the direction of propagation inducing a radial component to the EF.|
|Sham exposure||A sham exposure was conducted.|
|Additional information||Sham exposures were performed in an identical RTL without the RF field. The temperature of the culture medium was maintained at 37.0 ± 0.25°C by feedback controllers for each RTL.|
Exposure of cells to a FMCW or CDMA field did not result in any significant variation in Jun and Myc mRNA levels or the DNA-binding activities of AP1, AP2, and NFkB.
Thus it is concluded that it is unlikely that the radiofrequency field is capable of eliciting a general stress response in cells of this cell line. However, the statistically significant increase in Fos expression suggests that the radiation could affect expression of specific genes.