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To study the effects of radiofrequency fields, emitted by mobile phones, on gene expression in cultured human cell lines at 0 and 18 h after exposure.
All samples were compared to sham-exposed, negative (incubator) and positive (heat shock, 1 h at 43°C) controls at 0 and 18 h after exposure.
|ばく露時間||intermittent, 5 min on/10 min off, for 6 h|
|チャンバの詳細||The temperature in the cell cultures was maintained at 37.0 ± 0.5°C.|
|ばく露装置の詳細||Cells were exposed in 10 ml of culture medium in 60-mm Petri dishes placed atop circularly polarized cylindrical waveguide applicators.|
|Sham exposure||A sham exposure was conducted.|
|Additional information||Negative and positive (heat shock) controls were run concurrently with each experiment.|
No significant effects were observed in mRNA expression of hsp27, hsp70, c-jun, c-myc or c-fos in comparison of the sham-exposed and radiofrequency-exposed groups, in either of the two cell lines. However, the positive control displayed a significant elevation in the expression of hsp27, hsp70, c-fos and c-jun in both cell lines compared to the sham-exposed and negative control groups.
The data revealed no evidence that exposure of cells to non-thermal levels of 1.9 GHz pulse modulated radiofrequency fields can cause any detectable change in stress-related gene expression.